About Enhancer
| Enhancer ID: | E_02_0776 |
| Species: | human |
| Position : | chr12:47471429-47487762   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | -- |
| Pubmed ID: | 29149598 |
| Enhancer experiment: | ATAC-seq,ChIP-seq,Luciferase Reporter Assay,Transfection,CRISPR/Cas9 |
| Enhancer experiment description: | Using Assay for Transposase-Accessible Chromatin (ATAC)-seq (Buenrostro et al., 2013) and ChIP-seq for H3K4me1 and H3K27ac (Creyghton et al., 2010), we identified ~1,400 constituent enhancers within the SEs of melanoma cells (Table S5) and found them largely devoid of open chromatin, H3K27ac, and H3K4me1 in NHMs (Figures 5F and ?and5G).To test the functionality of the predicted enhancers (Figures 6B and ?and7A), we cloned E1E5 upstream of a minimal promoter driving luciferase expression (Prescott et al., 2015) and trans-fected these constructs into SKmel147 cells. We next used CRISPR/Cas9 editing to individually delete the genomic regions containing E2E3 and E4E5. |
About Target gene
| Target gene : | AMIGO2(ALI1,AMIGO-2,DEGA),AMIGO2(ALI1,AMIGO-2,DEGA) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | Luciferase Reporter Assay |
| Target gene experiment description: | Schematic of AMIGO2 SE showing enhancers E1E5 (B), deleted sequences for CRISPR-Cas9 editing (C), and DNA segments used for E3 luciferase assay (D). Luciferase reporter assays performed in SKmel147 cells for AMIGO2 enhancer elements E3AC and E3A deleted for TF motifs (E3A-Del) ;Schematic of AMIGO2 SE showing enhancers E1E5 (B), deleted sequences for CRISPR-Cas9 editing (C), and DNA segments used for E3 luciferase assay (D). Luciferase reporter assays performed in SKmel147 cells for AMIGO2 enhancer elements E3AC and E3A deleted for TF motifs (E3A-Del) |
About TF
| TF name : | FOSL2(FRA2)TEAD4(EFTR-2,RTEF1,TCF13L1,TEF-3,TEF3,TEFR-1,hRTEF-1B)FOSL2(FRA2)TEAD4(EFTR-2,RTEF1,TCF13L1,TEF-3,TEF3,TEFR-1,hRTEF-1B) |
| TF experiment: | Immunoblot |
| TF experiment description: | FOSL2 (left), TEAD4 (right), and AMIGO2 immunoblots of SKmel147 cells 96 hr post-transduction with shSCR or shFOSL2 (shF2 #1 and #2) and shSCR or shTEAD4+TEAD1 (shT4 #1+shT1 #1 and shT4 #2+shT1 #1). HSP90 and GAPDH were used as loading controls.;FOSL2 (left), TEAD4 (right), and AMIGO2 immunoblots of SKmel147 cells 96 hr post-transduction with shSCR or shFOSL2 (shF2 #1 and #2) and shSCR or shTEAD4+TEAD1 (shT4 #1+shT1 #1 and shT4 #2+shT1 #1). HSP90 and GAPDH were used as loading controls. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene