About Enhancer

Enhancer ID: E_02_0777
Species: human
Position : chr6:91004796-91006944
Biosample name:
Experiment class : low throughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  29129929
Enhancer experiment: RT-PCR,ChIP-qPCR,Luciferase Reporter Assay
Enhancer experiment description: DNA immunoprecipitated by total ELK1 antibody or immunoglobulin G (IgG CTL) was amplified by real-time PCR using primers flanking the putative ELK1 binding site in BACH2 intron 1 (predicted BACH2 enhancer position), known ELK1 binding regions in MCL1, MYD88 and FOS genes, and negative control primers as a reference (negative site). ChIP analysis of histone modifications and p300 binding in IL-2-primed D3 B cells. We demonstrated an in vivo binding of ELK1 to this putative enhancer by chromatin immunoprecipitation followed by QPCR (ChIP-QPCR) in IL2-primed B cells collected at D3.

About Target gene

Target gene : BACH2(BTBD25,IMD60)
Strong evidence: --
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: We decided to detail the functional DNA regulatory sequence by luciferase reporter assay in the context of primary human naive B cell activation. The BACH2 proximal promoter41 (minPBACH2, ?725; +146) ligated upstream from the nano-luciferase coding sequence (NanoLuc) demonstrated a 4.2??0.2 (n?=?15) fold more transcriptional activity than the promoterless NanoLuc vector (pNL1.1), whatever the time point of B-cell electroporation (from D0 to D3). The enhancer region (+1265; +1493) was inserted in the native and reverse orientation downstream from the minPBACH2.

About TF

TF name : ELK1(ELK1)
TF experiment: ChIP-qPCR
TF experiment description: ChIP assay of the in vivo binding of ELK1 in IL-2-primed D3 B cells. DNA immunoprecipitated by total ELK1 antibody or immunoglobulin G (IgG CTL) was amplified by real-time PCR using primers flanking the putative ELK1 binding site in BACH2 intron 1 (predicted BACH2 enhancer position), known ELK1 binding regions in MCL1, MYD88 and FOS genes, and negative control primers as a reference (negative site). Means s.e.m. of four independent experiments.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
ELK1 Angiopoietin receptor Tie2-mediated signaling pid 50
ELK1 Angiotensin_II-stimulated_signaling_through_G_proteins_and_beta-arrestin panther 34
ELK1 BCR netpath 161
ELK1 BCR signaling pathway pid 70
ELK1 Downstream signaling in nave CD8+ T cells pid 69
ELK1 EGFR1 netpath 475
ELK1 ErbB1 downstream signaling pid 105
ELK1 ERK cascade ( EGF signaling pathway Diagram ) inoh 6
ELK1 ERK cascade ( FGF signaling pathway ) inoh 8
ELK1 ERK cascade ( FGF8 signaling (Mouse) ) inoh 17
ELK1 ERK cascade ( FGF8 signaling (Xenopus) ) inoh 17
ELK1 ERK cascade ( HGF signaling pathway ) inoh 6
ELK1 ERK cascade ( IGF1 signaling pathway ) inoh 8
ELK1 ERK cascade ( Insulin receptor signaling (D. melanogaster) ) inoh 8
ELK1 ERK cascade ( Insulin receptor signaling (Mammal) ) inoh 8
ELK1 ERK cascade ( NGF signaling pathway ) inoh 8
ELK1 ERK cascade ( VEGF signaling pathway ) inoh 6
ELK1 ERK/MAPK targets reactome 17
ELK1 FGF8 signaling pathway(Mouse) ( FGF8 signaling (Mouse) ) inoh 20
ELK1 FGF8 signaling pathway(Xenopus) ( FGF8 signaling (Xenopus) ) inoh 18
ELK1 HIF-2-alpha transcription factor network pid 34
ELK1 ID netpath 38
ELK1 IL2 netpath 81
ELK1 IL4 netpath 75
ELK1 IL5 netpath 59
ELK1 Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade panther 29
ELK1 Interleukin signaling pathway panther 86
ELK1 Intracellular Signalling Through Adenosine Receptor A2a and Adenosine smpdb 37
ELK1 Intracellular Signalling Through Adenosine Receptor A2b and Adenosine smpdb 38
ELK1 KitReceptor netpath 104
ELK1 Leptin netpath 98
ELK1 Oxidative stress response panther 23
ELK1 p38 MAPK pathway panther 31
ELK1 Parkinson disease panther 85
ELK1 PDGF signaling pathway panther 113
ELK1 PDGFR-alpha signaling pathway pid 22
ELK1 PDGFR-beta signaling pathway pid 125
ELK1 Prolactin netpath 105
ELK1 Ras Pathway panther 69
ELK1 Ras signaling in the CD4+ TCR pathway pid 14
ELK1 S1P2 pathway pid 26
ELK1 Toll receptor signaling pathway panther 44
ELK1 Trk receptor signaling mediated by the MAPK pathway pid 34
ELK1 ERK cascade ( PDGF signaling pathway ) inoh 7
ELK1 MAPK signaling pathway kegg 264
ELK1 ErbB signaling pathway kegg 83
ELK1 Focal adhesion kegg 197
ELK1 Insulin signaling pathway kegg 134
ELK1 GnRH signaling pathway kegg 98
ELK1 Prion diseases kegg 34
ELK1 Leishmaniasis kegg 71
ELK1 Endometrial cancer kegg 52
ELK1 Hs_Cytokines_and_Inflammatory_Response_WP530_79331 wikipathways 21
ELK1 Hs_MAPK_Signaling_Pathway_WP382_79951 wikipathways 58
ELK1 Hs_Leptin_signaling_pathway_WP2034_89856 wikipathways 37
ELK1 Hs_Serotonin_Receptor_4-6-7_and_NR3C_Signaling_WP734_74438 wikipathways 15
ELK1 Hs_Structural_Pathway_of_Interleukin_1_(IL-1)_WP2637_87707 wikipathways 34
ELK1 Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
ELK1 Hs_G_Protein_Signaling_Pathways_WP35_88601 wikipathways 8
ELK1 Hs_Serotonin_Receptor_2_and_ELK-SRF-GATA4_signaling_WP732_80010 wikipathways 13
ELK1 Hs_IL-5_Signaling_Pathway_WP127_78498 wikipathways 35
ELK1 Hs_IL-4_Signaling_Pathway_WP395_89828 wikipathways 41
ELK1 Hs_Brain-Derived_Neurotrophic_Factor_(BDNF)_signaling_pathway_WP2380_89803 wikipathways 80
ELK1 Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107
ELK1 Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 wikipathways 71

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs