About Enhancer
| Enhancer ID: | E_02_0786 |
| Species: | mouse |
| Position : | chr11:109985003-109985222   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Osteosarcoma |
| Pubmed ID: | 28905448 |
| Enhancer experiment: | ChIP-PCR,Luciferase Reporter Assay |
| Enhancer experiment description: | Interestingly, mutation of the Sox2 binding site at mt 11, whose sequence is not con? served, had no effect. Mutations at positions m8, m14,and m15 strongly affected the luciferase activity of thereporter plasmid in both cell types. Overexpression of Sox2 did not significantly stimulate the m8, m14, and m15 mutant plasmid activity in 293 cells, indicating that these motifs are critical for enhancer activity. |
About Target gene
| Target gene : | YAP1(COB1,YAP,YAP2,YAP65,YKI) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-PCR |
| Target gene experiment description: | To further confirm the binding of MZF1 to the YAP1 enhancer region ChIPPCR was performed.The results showed binding of MZF1 to specific DNA sequences within the YAP1 enhancer region (Fig. 2C). |
About TF
| TF name : | MZF1(MZF-1B,ZFP98,ZNF42,ZSCAN6,MZF1)SOX2(ANOP3,MCOPS3) |
| TF experiment: | EMSA,Luciferase Reporter Assay |
| TF experiment description: | EMSA analysis of the binding of GABP, and MZF1 transcription factor to the YAP enhancer region;A reporter plasmid expressing luciferase under the control of the FGF4 minimal promoter and the YAP enhancer was transfected into 293 cells in combination with different plasmids expressing MZF1, Sox2 and GABP cDNA. |
About Function
| Enhancer function : | Osteogenic Differentiation |
| Enhancer function experiment: | CRISPR/Cas9,Alkaline Phosphatase Staining |
| Enhancer function experiment description: |
MZF1 binding to the YAP enhancer is essential for the endogenous expression of YAP;Osteogenic differentiation was detected using alkaline phosphatase staining |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SOX2 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| SOX2 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| SOX2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| SOX2 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| SOX2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| SOX2 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| SOX2 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
| SOX2 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| SOX2 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene