About Enhancer
| Enhancer ID: | E_02_0795 |
| Species: | mouse |
| Position : | chr9:88325101-88326201   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Hepatic fibrosis |
| Pubmed ID: | 28667437 |
| Enhancer experiment: | Luciferase Reporter Assay |
| Enhancer experiment description: | The measured luciferase activity noticeably decreased between F1- and F2-Luc constructs, suggesting that F1-Luc construct contains a potential enhancer sequence, which spans approximately 1000 bp (?2069 to ?996 bp) and is absent in the shorter F2-Luc construct. |
About Target gene
| Target gene : | Nt5e(CALJA,CD73,E5NT,NT,NT5,NTE,eN,eNT) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,PCR |
| Target gene experiment description: | Luciferase activity as_x0002_says showed that the ENH1074-F5-Luc construct containing the full putative Enhancer (-2069 to -996 bp) sequence has the ability to enhance the luciferase activity of the identified putative mouse Nt5e promoter. |
About TF
| TF name : | Elf2(2610036A20Rik,A230104O07Rik,AW111824,BB183398,EU32,NERF,NERF-1A,NERF-1B,NERF-2) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | We found that mutation of Elf2 responsive element alone was sufficient to significantly increase the F5-Luc construct luciferase activity (F5-Luc, 11.79 1.41 vs. mut Elf2-F5-Luc,20.48 2.96, **p < 0.01).These data indicate that repression of mouse Nt5e promoter activity is likely mediated by transcription factor(s) acting at the identified Elf2 site in NIH3T3 fibroblasts. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Elf2 | Angiopoietin receptor Tie2-mediated signaling | pid | 50 |
| Elf2 | PDGF signaling pathway | panther | 113 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene