About Enhancer
| Enhancer ID: | E_02_0797 |
| Species: | mouse |
| Position : | chr17:62627957-62633457   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 28614915 |
| Enhancer experiment: | Luciferase Reporter Assay |
| Enhancer experiment description: | Luciferase activity assay showing that Ascl1- E47 fusion protein induces transcriptional activation through the ephrin-A5 E-box DNA.Taken together, our findings suggest that the mesencephalon-specific enhancer of ephrin-A5 resided in a genomic region that is +11.6 kb to +57.7 kb from the TSS and is within the first intron (Fig. 1A). |
About Target gene
| Target gene : | Efna5(AL-1,AV158822,EFL-5,Ephrin-A5,Epl7,LERK-7,RAGS) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,PCR |
| Target gene experiment description: | To further restrict the possible locations of the mesencephalon-specific enhancer within the first intron, the 375B7 BAC was modified using a different targeting vector. Importantly, transgenic embryos carrying this recombinant BAC did not show significant LacZ expression in the mesencephalon (Fig. 2C, first panel from the left). This suggested that the deleted 30.4 kb region included the mesencephalon-specific enhancer of ephrin-A5.This suggests that the potential enhancer for ephrin-A5 expression in the mesencephalon was in a 5.5 kb genomic region from +25 kb to +30.5 kb of the TSS. |
About TF
| TF name : | Ascl1(AI225900,ASH1,Mash1,bHLHa46) |
| TF experiment: | EMSA,Luciferase Reporter Assay |
| TF experiment description: | Finally, both electrophoretic mobility shift assay and luciferase-based transactivation assay revealed that the transcription factor Ascl1 bound the bHLH consensus binding site in the mesencephalon-specific ephrin-A5 enhancer in vitro. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Ascl1 | Notch-mediated HES/HEY network | pid | 48 |
| Ascl1 | Notch | netpath | 76 |
| Ascl1 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| Ascl1 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene