About Enhancer
| Enhancer ID: | E_02_0813 |
| Species: | human |
| Position : | chr3:46988575-46990242   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 28082341 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,RT-PCR |
| Enhancer experiment description: | Chromatin immunoprecipitation sequencing revealed 5 GATA binding sites in a regulatory region 31 kb upstream of NBEAL2 covered by a H3K4Me1 mark indicative of an Enhancer locus.Luciferase reporter constructs containing this region confirmed its Enhancer activity in K562 cells, and mutagenesis of the GATA1 binding sites resulted in significantly reduced Enhancer activity. |
About Target gene
| Target gene : | NBEAL2(BDPLT4,GPS) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,ChIP,RT-PCR |
| Target gene experiment description: | Chromatin immunoprecipitation sequencing revealed 5 GATA binding sites in a regulatory region 31 kb upstream of NBEAL2 covered by a H3K4Me1 mark indicative of an Enhancer locus.Luciferase reporter constructs containing this region confirmed its Enhancer activity in K562 cells, and mutagenesis of the GATA1 binding sites resulted in significantly reduced Enhancer activity. |
About TF
| TF name : | GATA1(ERYF1,GATA-1,GF-1,GF1,NF-E1,NFE1,XLANP,XLTDA,XLTT) |
| TF experiment: | Immunoblot |
| TF experiment description: | Immunoblot analysis for NBEAL2 with a rabbit polyclonal antibody and integrin ?3 were performed (left) using total protein MK lysates at days 8 and 11 for the control and day 11 for GATA1 D218Y (right) using total protein MK lysates at day 13 for the control and GATA1 D218G. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| GATA1 | C-MYB transcription factor network | pid | 87 |
| GATA1 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA1 | IL3 | netpath | 84 |
| GATA1 | Notch-mediated HES/HEY network | pid | 48 |
| GATA1 | p73 transcription factor network | pid | 80 |
| GATA1 | Signaling events mediated by HDAC Class I | pid | 89 |
| GATA1 | Signaling events mediated by HDAC Class II | pid | 58 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene