About Enhancer
| Enhancer ID: | E_02_0814 |
| Species: | mouse |
| Position : | chr11:112769210-112772210   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 28045957 |
| Enhancer experiment: | Transgenic mice,CRISPR/Cas9,qRT-PCR |
| Enhancer experiment description: | During mouse sex determination, transient expression of the Y-linked gene Sry up-regulates its direct target gene Sox9, via a 3.2 kb testis specific enhancer of Sox9 (TES), which includes a core 1.4 kb element, TESCO. SOX9 activity leads to differentiation of Sertoli cells, rather than granulosa cells from the bipotential supporting cell precursor lineage. Here, we present functional analysis of TES/TESCO, using CRISPR/Cas9 genome editing in mice. Deletion of TESCO or TES reduced Sox9 expression levels in XY fetal gonads to 60 or 45% respectively relative to wild type gonads, and reduced expression of the SOX9 target Amh. |
About Target gene
| Target gene : | Sox9 (CMD1,CMPD1,SRA1,SRXX2,SRXY10),Sox9 (CMD1,CMPD1,SRA1,SRXX2,SRXY10) |
| Strong evidence: | -- |
| Less strong evidence: | qRT-PCR |
| Target gene experiment description: | These results of qRT-PCR clearly indicate that the TESCO Enhancer is an important regulator of Sox9 expression throughout testis development, accounting for around 40% of its expression levels.;These results of qRT-PCR clearly indicate that the TESCO Enhancer is an important regulator of Sox9 expression throughout testis development, accounting for around 40% of its expression levels. |
About TF
| TF name : | ---- |
| TF experiment: | -- |
| TF experiment description: | --;-- |
About Function
| Enhancer function : | reduced expression of the SOX9 target Amh. |
| Enhancer function experiment: | CRISPR/Cas9 |
| Enhancer function experiment description: |
Here, we present functional analysis of TES/TESCO, using CRISPR/Cas9 genome editing in mice. Deletion of TESCO or TES reduced Sox9 expression levels in XY fetal gonads to 60 or 45% respectively relative to wild type gonads, and reduced expression of the SOX9 target Amh. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene