About Enhancer
| Enhancer ID: | E_02_0816 |
| Species: | human |
| Position : | chr18:69656860-69683465   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | -- |
| Pubmed ID: | 27986456 |
| Enhancer experiment: | ATAC-seq |
| Enhancer experiment description: | ATAC-Seq analysis of Tcf4 locus in primary DCs. Shown are ATAC-Seq peaks across ~1.5 Mb of the Tcf4 locus; indicated are proximal promoters of Tcf4L (green) and Tcf4S (purple), the 3? enhancer (orange) and the cluster of 5? regulatory elements (yellow). In this and subsequent panels, the scale was adjusted between different samples based on signals from housekeeping genes.We analyzed the 3? region by ChIP during the differentiation of HoxB8-FL cells. Histone modifications associated with active enhancers, including histone 3 lysine 4 monomethylation (H3K4me1) and lysine 27 acetylation (H3K27ac), were induced in this region on day 4, i.e. at the onset of pDC differentiation (Fig. 5D). |
About Target gene
| Target gene : | Tcf4(5730422P05Rik,ASP-I2,E2-2,E2.2,ITF-2,ITF-2b,ITF2,ME2,MITF-2A,MITF-2B,SEF-2,SEF2,SEF2-1,TFE,Tcf-4,bHLHb19) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | ChIP-seq,ATAC-seq |
| Target gene experiment description: | ChIP analysis of histone modifications (H3K4me1 and H3K27ac) and of TCF4 binding to the 3? Enhancer in differentiating HoxB8-FL cells. |
About TF
| TF name : | Tcf4(E2-2,FECD3,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4,bHLHb19) |
| TF experiment: | qRT-PCR |
| TF experiment description: | By qRT-PCR, Tcf4S was prominently expressed in pDCs but also present in other cell types including B cells and cDCs. |
About Function
| Enhancer function : | A conserved Enhancer downstream of Tcf4 was required for its upregulation during pDC differentiation, revealing a positive feedback loop |
| Enhancer function experiment: | qRT-PCR,CRISPR/Cas9 |
| Enhancer function experiment description: |
By qRT-PCR, Tcf4S was prominently expressed in pDCs but also present in other cell types including B cells and cDCs |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Tcf4 | AndrogenReceptor | netpath | 167 |
| Tcf4 | CDO in myogenesis | reactome | 29 |
| Tcf4 | Coregulation of Androgen receptor activity | pid | 61 |
| Tcf4 | Formation of the beta-catenin:TCF transactivating complex | reactome | 88 |
| Tcf4 | ID | netpath | 38 |
| Tcf4 | Regulation of nuclear beta catenin signaling and target gene transcription | pid | 80 |
| Tcf4 | TGF_beta_Receptor | netpath | 220 |
| Tcf4 | Wnt | netpath | 118 |
| Tcf4 | Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 | wikipathways | 41 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene