About Enhancer
| Enhancer ID: | E_02_0817 |
| Species: | human |
| Position : | chr1:24601612-24626812   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Breast cancer |
| Pubmed ID: | 27980063 |
| Enhancer experiment: | ChIP-seq,Western blot |
| Enhancer experiment description: | ChIP analyses of BRD4 and RNAPII occupancy revealed that decreased BRD4 occupanc on enhancers in response to JQ1 or OTX015 treatment leads to a signiicant concomitant reduction in RNAPII occupancy on these regions (Figure 4H, I and K, L). While BRD4 occupancy was not significantly affected around the Np63 TSS, the potential enhancers displayed a substantial reduction in the occupancy of both BRD4 and RNAPII (Figure 4HandI). Most importantly, BRD4 inhibition resulted in the down regulation of the putative TP63-- and GRHL3--associated eRNAs (Figure 4J and N). |
About Target gene
| Target gene : | GRHL3(SOM,TFCP2L4,VWS2) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP |
| Target gene experiment description: | ChIP analyses of BRD4 and RNAPII occupancy revealed that decreased BRD4 occupanc on enhancers in response to JQ1 or OTX015 treatment leads to a significant concomitant reduction in RNAPII occupancy on these regions (Figure 4H, I and K, L).Altogether, these studies demonstrate an enhancer--associated function of BRD4 in modulating the expression of TP63 and GRHL3. |
About TF
| TF name : | FOXO(FOXO)GRHL3(SOM,TFCP2L4,VWS2) |
| TF experiment: | Western blot |
| TF experiment description: | Western blot analyses of MCF10A protein ysates showing total p63 and GRHL3 expression with 100 nM and 1 _x0002_M of FOXO1 inhibitor AS1842856 (FOXO1i) for 24 h after removal of EGF and Insulin (-EGF/-Ins) for 48 h. |
About Function
| Enhancer function : | This Enhancer is located in a region where a long-range chromatin interaction among the promoters and promoter-Enhancer of several genes has been described, possibly affecting their expression simultaneously. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
In the present work, by integrating several strategies, we have uncovered the variant most strongly associated with MS in the region. This variant also affects the activity of an Enhancer in an allele-dependent and orientation-dependent manner and correlates with the expression of five genes of the locus. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene