About Enhancer
| Enhancer ID: | E_02_0819 |
| Species: | mouse |
| Position : | chr5:137465620-137479420   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Renal anemia |
| Pubmed ID: | 27920250 |
| Enhancer experiment: | Luciferase Reporter Assay,Transgenic mice |
| Enhancer experiment description: | 17k-GFP contains the 17-kb upstream and 15-kb downstream regions, whereas 17kXM-GFP contains only the 17-kb upstream region as a regulatory element. As in 22k-GFP mice, GFP expression was observed in the kidney and liver in 17k-GFP mice under anemic conditions (Fig. 1C). |
About Target gene
| Target gene : | Epo(Epo) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,qRT-PCR |
| Target gene experiment description: | we generated a series of transgenic GFP reporter constructs using 60k-GFP and 22k-GFP as the starting materia GFP expression was observed in the kidney but not in the liver in 17kXM-GFP mice.By means of long genomic PCR assays utilizing a primer set recognizing sequences located 18.6 kb upstream (forward primer) and 3.7 kb downstream (reverse primer) from the Epo gene transcription start site, we confirmed that the bulk of the Epo-BAC DNA containing a 17-kb upstream region and a downstream hepatic enhancer region was integrated into the genome of the transgenic mice in the right order (Fig. 4C). |
About TF
| TF name : | -- |
| TF experiment: | -- |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene