| Target gene experiment description: |
We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B).;We found that the enhancer cluster EC(DppUp), which lies between the Dppa5a and Ooep genes, regulated both Dppa5a and Ooep in cis. We considered two alternatives for the mechanism underlying this observation: Either this region contained two distinct regulatory modules, one for Dppa5a and one for Ooep, or each of the enhancers within the cluster contributed to the expression of both genes. To differentiate between these two alternatives, we split the EC into two segments and deleted each individually (Fig. 2). The region closest to Ooep, EC(DppUpB), contains one MTL bound by four transcription factors, whereas the region closest to Dppa5a, EC(DppUpA), contains three separate MTLs. Reporter assays revealed that each of these regions contained an active enhancer (Fig. 2B). |
