About Enhancer
| Enhancer ID: | E_02_0822 |
| Species: | human |
| Position : | chr11:2219531-2239062   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer |
| Disease: | Lung cancer |
| Pubmed ID: | 27869826 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay |
| Enhancer experiment description: | We next performed ChIP-Seq at the IGF2 locus, detecting an accumulation of the active chromatin mark H3K27ac at a previously identified IGF2 enhancer66 herein referred to as IGF2 cognate enhancer (Fig. 5a, Supplementary Fig. 11). An even more pronounced H3K27ac peak, however, intersected with an element previously inferred to represent a lineage-specific super-enhancer in CRC cell lines (VACO-400 and VACO-9M)46.To verify enhancer function, we performed luciferase assays revealing enhancer activity of cloned fragments of this previously inferred super-enhancer46 in the HCT116 colon cancer but not in a control (HeLa) cancer cell line (Supplementary Fig. 12 and Supplementary Table 6). |
About Target gene
| Target gene : | IGF2(C11orf43,GRDF,IGF-II,PP9974) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay |
| Target gene experiment description: | To verify enhancer function, we performed luciferase assays revealing enhancer activity of cloned fragments of this previously inferred super-enhancer46 in the HCT116 colon cancer but not in a control (HeLa) cancer cell line (Supplementary Fig. 12 and Supplementary Table 6). |
About TF
| TF name : | -- |
| TF experiment: | -- |
| TF experiment description: | -- |
About Function
| Enhancer function : | this IGF2 region indeed interacts with and hence represents a candidate IGF2 Enhancer. |
| Enhancer function experiment: | 4C-seq |
| Enhancer function experiment description: |
we additionally performed 4C-Seq (chromosome conformation capture sequencing63) experiments employing the putative CRE downstream of IRS4 as a viewpoint |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene