About Enhancer
| Enhancer ID: | E_02_0823 |
| Species: | human |
| Position : | chr1:183149939-183150336   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 27333824 |
| Enhancer experiment: | Luciferase Reporter Assay |
| Enhancer experiment description: | To determine whether CDX2 regulates the basal LAMC2 gene activity, a luciferase reporter gene driven by the LAMC2 promoter with and without the upstream LAMC2 enhancer identi?ed previously [Boyd et al., 2014] was transfected into Caco-2 cells without or with concomitant CDX2 expression plasmid. Over-expression of CDX2 increased the luciferase activity driven by the enhancer-less construct of LAMC2 by 2.5-fold (P?0.028) relative to cells transfected with the LAMC2 reporter vector alone, whereas the LAMC2 promoter with enhancer construct gave a fourfold increase (P?0.006) in reporter signal when co-transfected with CDX2 expression plasmid relative to cells transfected with the LAMC2 promoter/enhancer reporter vector alone (Fig. 1A). The LAMC2 luciferase reporter constructs were: pGL3-LAMC2 containing the 1.2 kb human LAMC2 promoter region upstream of transcription start site [Olsen et al., 2000], and pGL3-LAMC2+ Enhancer containing the 1.2 kb LAMC2 promoter and the enhancer region at chr1:183,149,939183,150,336 (hg19) [Boyd et al., 2014]. |
About Target gene
| Target gene : | LAMC2(B2T,BM600,CSF,EBR2,EBR2A,LAMB2T,LAMNB2) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay |
| Target gene experiment description: | To determine whether CDX2 regulates the basal LAMC2 gene activity, a luciferase reporter gene driven by the LAMC2 promoter with and without the upstream LAMC2 enhancer identi?ed previously [Boyd et al., 2014] was transfected into Caco-2 cells without or with concomitant CDX2 expression plasmid. Over-expression of CDX2 increased the luciferase activity driven by the enhancer-less construct of LAMC2 by 2.5-fold (P?0.028) relative to cells transfected with the LAMC2 reporter vector alone, whereas the LAMC2 promoter with enhancer construct gave a fourfold increase (P?0.006) in reporter signal when co-transfected with CDX2 expression plasmid relative to cells transfected with the LAMC2 promoter/enhancer reporter vector alone (Fig. 1A). |
About TF
| TF name : | CDX2(CDX-3/AS,CDX3,CDX2) |
| TF experiment: | ChIP-seq |
| TF experiment description: | Thus, to further analyze,whether LAMC2 is a direct target of CDX2 also in the native chromatin environment, we performed ChIP experiments with Caco-2 and with cells purified from human colonic tissue samples. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| CDX2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| CDX2 | Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) | reactome | 20 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene