About Enhancer
| Enhancer ID: | E_02_0824 |
| Species: | human |
| Position : | chr11:95817801-95818824   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Breast cancer |
| Pubmed ID: | 27207652 |
| Enhancer experiment: | ChIP,qPCR,Transfection,Western blot |
| Enhancer experiment description: | The difference in DNA meth_x0002_ylation within MAML2 in RSV treated versus control cells was determined as 0.37 in MCF10CA1h and 0.1 in MCF10CA1a cells based on the array data. These elevated levels of methylation were identified in a fragment corresponding to the predic_x0002_tive enhancer within MAML2 gene body. It has been demonstrated that active enhancers are enriched with H3K27ac.We confirmed by ChIP assay that this histone mark is present at MAML2 in cancer cells and its occupancy is significantly reduced by RSV.These data strongly support the role of this region as a gene enhancer and the role of RSV in decreasing the activ_x0002_ity of this enhancer. |
About Target gene
| Target gene : | MAML2(MAM-3,MAM2,MAM3,MLL-MAML2) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,Transfection,Western blot |
| Target gene experiment description: | MAML2, a coactivator of NOTCH targets, is methylated at the enhancer region and transcriptionally silenced in response to stilbenoids, possibly explaining the downregulation of NOTCH target genes.It has been demonstrated that active enhancers are enriched with H3K27ac.We confirmed by ChIP assay that this histone mark is present at MAML2 in cancer cells and its occupancy is significantly reduced by RSV. |
About TF
| TF name : | POU2F1(OCT1,OTF1,oct-1B) |
| TF experiment: | ChIP,qPCR |
| TF experiment description: | Using ChIP, we confirmed OCT1 binding to MAML2 enhancer (Figure?6C, control cells). The binding dramati_x0002_cally decreases upon treatment of MCF10CA1a with 15??M RSV.The lack of OCT1 binding may be directly linked to MAML2 transcriptional silencing. To test this hypothesis, we depleted OCT1 in MCF10CA1a cells using SmartPool siRNA mixture and four siRNA sequences separately, as confirmed by QPCR and western blot and tested the effect of OCT1 absence on MAML2 expression. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| POU2F1 | AndrogenReceptor | netpath | 167 |
| POU2F1 | ATF-2 transcription factor network | pid | 59 |
| POU2F1 | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| POU2F1 | Calcium signaling in the CD4+ TCR pathway | pid | 29 |
| POU2F1 | FOXA1 transcription factor network | pid | 45 |
| POU2F1 | Glucocorticoid receptor regulatory network | pid | 85 |
| POU2F1 | Regulation of Androgen receptor activity | pid | 54 |
| POU2F1 | RNA polymerase II transcribes snRNA genes | reactome | 70 |
| POU2F1 | RNA Polymerase III Abortive And Retractive Initiation | reactome | 41 |
| POU2F1 | RNA Polymerase III Transcription Initiation From Type 3 Promoter | reactome | 28 |
| POU2F1 | Hs_Integrated_Cancer_Pathway_WP1971_82939 | wikipathways | 33 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene