About Enhancer
| Enhancer ID: | E_02_0825 |
| Species: | human |
| Position : | chr8:120876144-120876856   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 27166834 |
| Enhancer experiment: | 3C,ChIP-seq |
| Enhancer experiment description: | To identify the presence of physical interaction between the 11 enhancer candidates and the Foxf1 promoter, 3C assays were performed. Among 11 selected regions, three enhancer candidates demonstrated interactions with the Foxf1 promoter. |
About Target gene
| Target gene : | Foxf1(ACDMPV,FKHL5,FREAC1) |
| Strong evidence: | 3C |
| Less strong evidence: | RT-qPCR,DNaseI-seq,Transgenic mice |
| Target gene experiment description: | To investigate the in vivo activity of the discovered enhanc_x0002_ers and compare the location of activity with Foxf1-express_x0002_ing regions, we generated enhancer-driven lacZ-reporter mice 5?e207mFoxf1-lacZ and 5?e201mFoxf1-lacZ.Foxf1 mRNA was expressed in the maxilla, mandible, limbs, sclerotome and gut of wild-type mouse embryos at E12 (Fig. 2a), whereas lacZ-positive cells were observed in part of the forebrain and neural tube in 5?e207mFoxf1-lacZ transgenic mice at E11 and E12 as well as 5?e201mFoxf1-lacZ transgenic mouse embryos at E11 after x-gal staining.Foxf1 was also expressed in the mesenchyme surrounding epithelial buds in lungs at E12. |
About TF
| TF name : | Gli1(AV235269,Zfp-5,Zfp5)Gli2(Gli2)Gli3 |
| TF experiment: | RT-qPCR,Luciferase Reporter Assay |
| TF experiment description: | Schematic drawing of luciferase reporter constructs containing 112 bp 5?e207mFoxf1 and 173 bp 5?e201mFoxf1 fragments with Gli binding sites is shown. Luciferase reporter constructs were assayed for luciferase activity without or with Gli1 over-expression vector at 50 or 100 ng. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene