About Enhancer
| Enhancer ID: | E_02_0832 |
| Species: | human |
| Position : | chr9:14538685-14540685   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Adenoid cystic carcinoma |
| Pubmed ID: | 26829750 |
| Enhancer experiment: | Luciferase Reporter Assay,RT-PCR,ChIP,ChIP-seq |
| Enhancer experiment description: | Here we identify super-enhancer translocations that drive overexpression of the oncogenic transcription factor MYB as a recurrent theme in adenoid cystic carcinoma (ACC). Whole-genome sequencing data and chromatin maps highlight distinct chromosomal rearrangements that juxtapose super-enhancers to the MYB locus. Chromosome conformation capture confirms that the translocated enhancers interact with the MYB promoter. |
About Target gene
| Target gene : | NFIB(CTF,HMGIC/NFIB,MACID,NF-I/B,NF1-B,NFI-B,NFI-RED,NFIB2,NFIB3) |
| Strong evidence: | 3C |
| Less strong evidence: | ChIP,ChIP-seq |
| Target gene experiment description: | To test whether specific enhancers within the translocated super_x0002_enhancers might activate the MYB promoter, we examined their physical proximity to the promoter using chromosome conforma_x0002_tion capture (3C). First, we examined an ACC with a translocation involving MYB and the NFIB locus. |
About TF
| TF name : | TP63 (AIS,B(p51A),B(p51B),EEC3,KET,LMS,NBP,OFC8,RHS,SHFM4,TP53CP,TP53L,TP73L,p40,p51,p53CP,p63,p73H,p73L) |
| TF experiment: | ChIP-seq |
| TF experiment description: | To directly test whether TP63 co-binds with MYB, we mapped binding of this transcription factor by ChIP-seq.Remarkably, we found that 81% of TP63-binding sites in ACC were co-bound by MYB.These data suggest that MYB,TP63 and Notch signaling may coordinately orchestrate the diverse expression programs in ACC. |
About Function
| Enhancer function : | Here we identify the juxtaposition of super-enhancer regions to the MYB locus as the unifying feature of ACC translocations.Detailed genomic and epigenomic analyses of ACCs identify alternate rearrangements that translocate super-enhancers in the NFIB and |
| Enhancer function experiment: | 3C |
| Enhancer function experiment description: |
To test whether specific enhancers within the translocated super_x0002_enhancers might activate the MYB promoter, we examined their physical proximity to the promoter using chromosome conforma_x0002_tion capture (3C).These data suggest that the translocations reposition super-enhancers that subsequently loop to the MYB promoter and sustain high-level MYB expression. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene