About Enhancer
| Enhancer ID: | E_02_0834 |
| Species: | mouse |
| Position : | chr2:10136078-10138078   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 26808502 |
| Enhancer experiment: | CRISPR/Cas9,ChIP,RT-PCR |
| Enhancer experiment description: | We next asked whether this 1.2-kb Gata3 Tce1 fragment was also necessary for the transcription of a reporter gene in T cells. To answer this question, we prepared another EGFP reporter plasmid in which the 1.2-kb sequence was deleted from full-length Tce1 (Figure 5A) and generated additional F0 Tg mice (Tg?1.2 mice). None of the F0 Tg?1.2 mice expressed EGFP in peripheral CD4 T cells (Table 1 and Supplemental Figure 5C) or in thymocytes (Figure 5, B and C), except possibly at the DN4 stage (Table 2). We also analyzed Tg?1.7, Tg?1.5, and Tg?2.7 mice and found that all of them expressed EGFP in peripheral CD4 T cells (Tables 1 and ?and2).2). Therefore, the EGFP expression in those mice confirmed that these sequences were not required for Tce1 enhancer activity in peripheral CD4 T cells. These data show that the 1.2-kb T cell enhancer fragment within Tce1 is also necessary for reporter gene transcription in T cells and, taken together with the data shown in Figure 1, that this fragment functions as an enhancer core element in vivo for Gata3 T cellspecific transcription. |
About Target gene
| Target gene : | Gata3(Gata-3,jal) |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | ChIP,PCR |
| Target gene experiment description: | As anticipated, homozygous loss of Tce1 (Tce1/) led to a reduction in the number of ETPs (62% of that of heterozygous controls), and Gata3 mRNA in the remaining ETPs was only 52% of that of controls (Figure 1B). In contrast, the number of Tce1/ DN2 and DN3 stage cells was unaltered compared with that of controls, although Gata3 mRNA levels in DN2 thymocytes were reduced (83% of that of heterozygous controls; Figure 1B). |
About TF
| TF name : | Tcf7(AI465550,TCF-1,Tcf1)Tcf12(A130037E08Rik,ALF1,HEB,HEBAlt,HTF-4,HTF4,ME1,REB,bHLHb20)Rbpj(AOS3,CBF1,IGKJRB,IGKJRB1,KBF2,RBP-JK,RBPSUH,SUH,csl,RBPJ) |
| TF experiment: | ChIP |
| TF experiment description: | We therefore examined the association of CSL/RBP-J with predicted binding sites within Tce1. CSL/RBP-J binding to the Cd25 locus, a robust direct Notch target gene, was used as a positive control with an antiRBP-J antibody in ChIP assays.Taken together, these data show that at least 3 critical T cellaffiliated transcription factors, TCF-1, HEB, and CSL/RBP-J, occupy binding sites within Tce1 at different developmental stages.Since all 3 have been shown to vitally affect T cell development, it seems likely that these factors and their associated signaling pathways directly modulate Gata3 expression through their binding to multiple consensus sites within Tce1. |
About Function
| Enhancer function : | Together, our data show that Tce1 is both necessary and sufficient for critical aspects of Gata3 T cellspecific transcriptional activity. |
| Enhancer function experiment: | CRISPR/Cas9,ChIP,RT-PCR |
| Enhancer function experiment description: |
Instead, we demonstrated that multiple lineage-affiliated transcription factors bind to Tce1 and that this enhancer confers T lymphocytespecific Gata3 activation in vivo, as targeted deletion of Tce1 in a mouse model abrogated critical functions of this T cellregulatory element. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene