About Enhancer

Enhancer ID: E_02_0838
Species: human
Position : chr6:36640937-36642937
Biosample name:
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: Tumour
Pubmed ID:  26745862
Enhancer experiment: ChIP,qPCR
Enhancer experiment description: Our ChIP experiments showed that prior to activation p53 was exclusively found at the upstream enhancer that harbors a high-affinity, near-consen_x0002_sus binding site for p53 (Fig 5A). Occupancy of this site increased incrementally after 1.5 and 4 hours of CDK9 inhibition (Fig 5B). In contrast to this pre-loaded "primary" enhancer, treat_x0002_ment with 067 was followed by de novo recruitment of p53 to additional, secondary binding sites, most notably to an intronic region that maps to position +4 kb downstream of the p21 TSS and contains another near-consensus p53 motif (Fig 5A). After four hours of treatment,p53 occupancy of this site reached similar levels as found at the upstream enhancer (Fig 5B).

About Target gene

Target gene : CDKN1A(CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1),CDKN1A(CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1)
Strong evidence: --
Less strong evidence: ChIP-qPCR
Target gene experiment description: Our ChIP experiments showed that prior to activation p53 was exclusively found at the upstream enhancer that harbors a high-affinity, near-consen_x0002_sus binding site for p53 (Fig 5A). Occupancy of this site increased incrementally after 1.5 and 4 hours of CDK9 inhibition (Fig 5B). In contrast to this pre-loaded "primary" enhancer, treat_x0002_ment with 067 was followed by de novo recruitment of p53 to additional, secondary binding sites, most notably to an intronic region that maps to position +4 kb downstream of the p21 TSS and contains another near-consensus p53 motif (Fig 5A). After four hours of treatment,p53 occupancy of this site reached similar levels as found at the upstream enhancer (Fig 5B).;Our ChIP experiments showed that prior to activation p53 was exclusively found at the upstream enhancer that harbors a high-affinity, near-consen_x0002_sus binding site for p53 (Fig 5A). Occupancy of this site increased incrementally after 1.5 and 4 hours of CDK9 inhibition (Fig 5B). In contrast to this pre-loaded "primary" enhancer, treat_x0002_ment with 067 was followed by de novo recruitment of p53 to additional, secondary binding sites, most notably to an intronic region that maps to position +4 kb downstream of the p21 TSS and contains another near-consensus p53 motif (Fig 5A). After four hours of treatment,p53 occupancy of this site reached similar levels as found at the upstream enhancer (Fig 5B).

About TF

TF name : TP53(BCC7,BMFS5,LFS1,P53,TRP53)TP53(BCC7,BMFS5,LFS1,P53,TRP53)
TF experiment: qPCR
TF experiment description: MCF7 cells, which harbour functional wild-type p53, were treated for 20 minutes to 4 hours with 10 ?M 067 and both nascent unspliced precursor (pre) and mature spliced mRNA was analyzed by quantitative PCR (qPCR). Inhibitor treatment led to significant(2.5-fold) repression of nascent RNA synthesis of the p53 target gene p21 within 20 minutes (Fig1A).;MCF7 cells, which harbour functional wild-type p53, were treated for 20 minutes to 4 hours with 10 ?M 067 and both nascent unspliced precursor (pre) and mature spliced mRNA was analyzed by quantitative PCR (qPCR). Inhibitor treatment led to significant(2.5-fold) repression of nascent RNA synthesis of the p53 target gene p21 within 20 minutes (Fig1A).

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
TP53 Activation of PUMA and translocation to mitochondria reactome 8
TP53 AP-1 transcription factor network pid 71
TP53 Apoptosis signaling pathway panther 104
TP53 Association of TriC/CCT with target proteins during biosynthesis reactome 39
TP53 Aurora A signaling pid 33
TP53 Autodegradation of the E3 ubiquitin ligase COP1 reactome 51
TP53 BARD1 signaling events pid 29
TP53 Direct p53 effectors pid 141
TP53 DNA Damage/Telomere Stress Induced Senescence reactome 64
TP53 EGFR1 netpath 475
TP53 Factors involved in megakaryocyte development and platelet production reactome 111
TP53 Formation of Senescence-Associated Heterochromatin Foci (SAHF) reactome 17
TP53 G2/M Checkpoints reactome 50
TP53 G2/M DNA damage checkpoint reactome 84
TP53 Glucocorticoid receptor regulatory network pid 85
TP53 Huntington disease panther 113
TP53 Hypoxic and oxygen homeostasis regulation of HIF-1-alpha pid 19
TP53 LKB1 signaling events pid 43
TP53 Oncogene Induced Senescence reactome 30
TP53 Oxidative Stress Induced Senescence reactome 120
TP53 p53 pathway by glucose deprivation panther 21
TP53 p53 pathway panther 39
TP53 p53 pathway pid 59
TP53 P53 pathway feedback loops 1 panther 6
TP53 p53 pathway feedback loops 2 panther 42
TP53 p75(NTR)-mediated signaling pid 74
TP53 PLK3 signaling events pid 6
TP53 Pre-NOTCH Transcription and Translation reactome 29
TP53 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks reactome 75
TP53 Regulation of TP53 Activity through Acetylation reactome 23
TP53 Regulation of TP53 Activity through Association with Co-factors reactome 14
TP53 Regulation of TP53 Activity through Methylation reactome 19
TP53 Regulation of TP53 Activity through Phosphorylation reactome 89
TP53 Regulation of TP53 Degradation reactome 35
TP53 Signaling events mediated by HDAC Class III pid 41
TP53 Signaling mediated by p38-alpha and p38-beta pid 35
TP53 Stabilization of p53 reactome 9
TP53 SUMOylation of transcription factors reactome 14
TP53 TGF_beta_Receptor netpath 220
TP53 The role of GTSE1 in G2/M progression after G2 checkpoint reactome 59
TP53 TNFalpha netpath 274
TP53 TP53 Regulates Metabolic Genes reactome 85
TP53 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain reactome 21
TP53 TP53 Regulates Transcription of Caspase Activators and Caspases reactome 12
TP53 TP53 Regulates Transcription of Death Receptors and Ligands reactome 12
TP53 TP53 Regulates Transcription of DNA Repair Genes reactome 61
TP53 TP53 Regulates Transcription of Genes Involved in Cytochrome C Release reactome 19
TP53 TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest reactome 13
TP53 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest reactome 18
TP53 TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain reactome 14
TP53 Validated targets of C-MYC transcriptional activation pid 80
TP53 Vinblastine Action Pathway smpdb 11
TP53 Vincristine Action Pathway smpdb 10
TP53 Vindesine Action Pathway smpdb 10
TP53 Vinorelbine Action Pathway smpdb 11
TP53 MAPK signaling pathway kegg 264
TP53 Cell cycle kegg 124
TP53 p53 signaling pathway kegg 66
TP53 Apoptosis kegg 88
TP53 Wnt signaling pathway kegg 135
TP53 Neurotrophin signaling pathway kegg 126
TP53 Amyotrophic lateral sclerosis (ALS) kegg 53
TP53 Huntington's disease kegg 182
TP53 Hepatitis C kegg 134
TP53 Pathways in cancer kegg 321
TP53 Colorectal cancer kegg 63
TP53 Pancreatic cancer kegg 70
TP53 Endometrial cancer kegg 52
TP53 Glioma kegg 65
TP53 Prostate cancer kegg 85
TP53 Thyroid cancer kegg 25
TP53 Basal cell carcinoma kegg 51
TP53 Melanoma kegg 69
TP53 Bladder cancer kegg 38
TP53 Chronic myeloid leukemia kegg 69
TP53 Small cell lung cancer kegg 83
TP53 Non-small cell lung cancer kegg 54
TP53 Hs_Cell_Cycle_WP179_89516 wikipathways 33
TP53 Hs_Tryptophan_metabolism_WP465_85055 wikipathways 45
TP53 Hs_ErbB_Signaling_Pathway_WP673_91869 wikipathways 37
TP53 Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974 wikipathways 36
TP53 Hs_TP53_Network_WP1742_71700 wikipathways 10
TP53 Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
TP53 Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
TP53 Hs_Nuclear_Receptors_in_Lipid_Metabolism_and_Toxicity_WP299_89331 wikipathways 10
TP53 Hs_Gastric_Cancer_Network_2_WP2363_87523 wikipathways 28
TP53 Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 wikipathways 36
TP53 Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 wikipathways 62
TP53 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
TP53 Hs_Apoptosis_Modulation_and_Signaling_WP1772_91293 wikipathways 71
TP53 Hs_Alzheimers_Disease_WP2059_87372 wikipathways 24
TP53 Hs_Copper_homeostasis_WP3286_89205 wikipathways 15
TP53 Hs_Integrated_Cancer_Pathway_WP1971_82939 wikipathways 33
TP53 Hs_ATM_Signaling_Pathway_WP2516_90247 wikipathways 32
TP53 Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 wikipathways 50
TP53 Hs_Pathways_Affected_in_Adenoid_Cystic_Carcinoma_WP3651_89271 wikipathways 27
TP53 Hs_LncRNA-mediated_mechanisms_of_therapeutic_resistance_WP3672_87395 wikipathways 9
TP53 Hs_Bladder_Cancer_WP2828_89143 wikipathways 17
TP53 Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520 wikipathways 16
TP53 Hs_Steroid_Biosynthesis_WP496_69016 wikipathways 8
TP53 Hs_Retinoblastoma_(RB)_in_Cancer_WP2446_87639 wikipathways 5
TP53 Hs_G1_to_S_cell_cycle_control_WP45_80001 wikipathways 8
TP53 Activation of PUMA and translocation to mitochondria reactome 8
TP53 AP-1 transcription factor network pid 71
TP53 Apoptosis signaling pathway panther 104
TP53 Association of TriC/CCT with target proteins during biosynthesis reactome 39
TP53 Aurora A signaling pid 33
TP53 Autodegradation of the E3 ubiquitin ligase COP1 reactome 51
TP53 BARD1 signaling events pid 29
TP53 Direct p53 effectors pid 141
TP53 DNA Damage/Telomere Stress Induced Senescence reactome 64
TP53 EGFR1 netpath 475
TP53 Factors involved in megakaryocyte development and platelet production reactome 111
TP53 Formation of Senescence-Associated Heterochromatin Foci (SAHF) reactome 17
TP53 G2/M Checkpoints reactome 50
TP53 G2/M DNA damage checkpoint reactome 84
TP53 Glucocorticoid receptor regulatory network pid 85
TP53 Huntington disease panther 113
TP53 Hypoxic and oxygen homeostasis regulation of HIF-1-alpha pid 19
TP53 LKB1 signaling events pid 43
TP53 Oncogene Induced Senescence reactome 30
TP53 Oxidative Stress Induced Senescence reactome 120
TP53 p53 pathway by glucose deprivation panther 21
TP53 p53 pathway panther 39
TP53 p53 pathway pid 59
TP53 P53 pathway feedback loops 1 panther 6
TP53 p53 pathway feedback loops 2 panther 42
TP53 p75(NTR)-mediated signaling pid 74
TP53 PLK3 signaling events pid 6
TP53 Pre-NOTCH Transcription and Translation reactome 29
TP53 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks reactome 75
TP53 Regulation of TP53 Activity through Acetylation reactome 23
TP53 Regulation of TP53 Activity through Association with Co-factors reactome 14
TP53 Regulation of TP53 Activity through Methylation reactome 19
TP53 Regulation of TP53 Activity through Phosphorylation reactome 89
TP53 Regulation of TP53 Degradation reactome 35
TP53 Signaling events mediated by HDAC Class III pid 41
TP53 Signaling mediated by p38-alpha and p38-beta pid 35
TP53 Stabilization of p53 reactome 9
TP53 SUMOylation of transcription factors reactome 14
TP53 TGF_beta_Receptor netpath 220
TP53 The role of GTSE1 in G2/M progression after G2 checkpoint reactome 59
TP53 TNFalpha netpath 274
TP53 TP53 Regulates Metabolic Genes reactome 85
TP53 TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain reactome 21
TP53 TP53 Regulates Transcription of Caspase Activators and Caspases reactome 12
TP53 TP53 Regulates Transcription of Death Receptors and Ligands reactome 12
TP53 TP53 Regulates Transcription of DNA Repair Genes reactome 61
TP53 TP53 Regulates Transcription of Genes Involved in Cytochrome C Release reactome 19
TP53 TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest reactome 13
TP53 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest reactome 18
TP53 TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain reactome 14
TP53 Validated targets of C-MYC transcriptional activation pid 80
TP53 Vinblastine Action Pathway smpdb 11
TP53 Vincristine Action Pathway smpdb 10
TP53 Vindesine Action Pathway smpdb 10
TP53 Vinorelbine Action Pathway smpdb 11
TP53 MAPK signaling pathway kegg 264
TP53 Cell cycle kegg 124
TP53 p53 signaling pathway kegg 66
TP53 Apoptosis kegg 88
TP53 Wnt signaling pathway kegg 135
TP53 Neurotrophin signaling pathway kegg 126
TP53 Amyotrophic lateral sclerosis (ALS) kegg 53
TP53 Huntington's disease kegg 182
TP53 Hepatitis C kegg 134
TP53 Pathways in cancer kegg 321
TP53 Colorectal cancer kegg 63
TP53 Pancreatic cancer kegg 70
TP53 Endometrial cancer kegg 52
TP53 Glioma kegg 65
TP53 Prostate cancer kegg 85
TP53 Thyroid cancer kegg 25
TP53 Basal cell carcinoma kegg 51
TP53 Melanoma kegg 69
TP53 Bladder cancer kegg 38
TP53 Chronic myeloid leukemia kegg 69
TP53 Small cell lung cancer kegg 83
TP53 Non-small cell lung cancer kegg 54
TP53 Hs_Cell_Cycle_WP179_89516 wikipathways 33
TP53 Hs_Tryptophan_metabolism_WP465_85055 wikipathways 45
TP53 Hs_ErbB_Signaling_Pathway_WP673_91869 wikipathways 37
TP53 Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974 wikipathways 36
TP53 Hs_TP53_Network_WP1742_71700 wikipathways 10
TP53 Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
TP53 Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
TP53 Hs_Nuclear_Receptors_in_Lipid_Metabolism_and_Toxicity_WP299_89331 wikipathways 10
TP53 Hs_Gastric_Cancer_Network_2_WP2363_87523 wikipathways 28
TP53 Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 wikipathways 36
TP53 Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 wikipathways 62
TP53 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
TP53 Hs_Apoptosis_Modulation_and_Signaling_WP1772_91293 wikipathways 71
TP53 Hs_Alzheimers_Disease_WP2059_87372 wikipathways 24
TP53 Hs_Copper_homeostasis_WP3286_89205 wikipathways 15
TP53 Hs_Integrated_Cancer_Pathway_WP1971_82939 wikipathways 33
TP53 Hs_ATM_Signaling_Pathway_WP2516_90247 wikipathways 32
TP53 Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 wikipathways 50
TP53 Hs_Pathways_Affected_in_Adenoid_Cystic_Carcinoma_WP3651_89271 wikipathways 27
TP53 Hs_LncRNA-mediated_mechanisms_of_therapeutic_resistance_WP3672_87395 wikipathways 9
TP53 Hs_Bladder_Cancer_WP2828_89143 wikipathways 17
TP53 Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520 wikipathways 16
TP53 Hs_Steroid_Biosynthesis_WP496_69016 wikipathways 8
TP53 Hs_Retinoblastoma_(RB)_in_Cancer_WP2446_87639 wikipathways 5
TP53 Hs_G1_to_S_cell_cycle_control_WP45_80001 wikipathways 8

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs