About Enhancer
| Enhancer ID: | E_02_0841 |
| Species: | mouse |
| Position : | chr14:54227135-54228033   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 26673693 |
| Enhancer experiment: | ChIP-seq,ChIP |
| Enhancer experiment description: | We found that eRNAs essentially reca_x0002_pitulated Pol II patterns during DN to DP transition as wellas those of Ets1 in DP only, suggesting highest enhancer ac_x0002_tivity in class 4 at the DN stage and classes 45 at the DP stage (Figure 3C). Thus, distal Ets1 enhancers broadly re_x0002_cruit Pol II that is able to generate paused transcripts.Significance of gene expression differences by increasing Ets1-fold change. Expression fold changes for all classes, shown as a boxplot. Significance levels for each comparison are indicated above, with 1 star denoting P < 0.05, 2 stars denoting P < 0.01 and 3 stars denoting P < 0.005. |
About Target gene
| Target gene : | Dad1(AI323713),Dad1(AI323713),Dad1(AI323713),Dad1(AI323713),Dad1(AI323713),Dad1(AI323713) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,Western blot,ChIP-qPCR |
| Target gene experiment description: | We further validated the dy_x0002_namic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.UCSC genome browser screenshot of the Tcra (left) and Cd2 loci (right)showing increased H3K4me1 signal at the Ets1 binding site following knockdown in the P5424 cell line.;We further validated the dy_x0002_namic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.UCSC genome browser screenshot of the Tcra (left) and Cd2 loci (right)showing increased H3K4me1 signal at the Ets1 binding site following knockdown in the P5424 cell line.;We further validated the dy_x0002_namic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.UCSC genome browser screenshot of the Tcra (left) and Cd2 loci (right)showing increased H3K4me1 signal at the Ets1 binding site following knockdown in the P5424 cell line.;We further validated the dy_x0002_namic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.UCSC genome browser screenshot of the Tcra (left) and Cd2 loci (right)showing increased H3K4me1 signal at the Ets1 binding site following knockdown in the P5424 cell line.;We further validated the dy_x0002_namic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.UCSC genome browser screenshot of the Tcra (left) and Cd2 loci (right)showing increased H3K4me1 signal at the Ets1 binding site following knockdown in the P5424 cell line.;We further validated the dy_x0002_namic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.UCSC genome browser screenshot of the Tcra (left) and Cd2 loci (right)showing increased H3K4me1 signal at the Ets1 binding site following knockdown in the P5424 cell line. |
About TF
| TF name : | Ets1(ETS-1,EWSR2,c-ets-1,p54)Tcf1Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)Ets1(ETS-1,EWSR2,c-ets-1,p54)Tcf1Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)Ets1(ETS-1,EWSR2,c-ets-1,p54)Tcf1Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)Ets1(ETS-1,EWSR2,c-ets-1,p54)Tcf1Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)Ets1(ETS-1,EWSR2,c-ets-1,p54)Tcf1Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha)Ets1(ETS-1,EWSR2,c-ets-1,p54)Tcf1Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha) |
| TF experiment: | ChIP-qPCR,Western blot |
| TF experiment description: | We further validated the dynamic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.;We further validated the dynamic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.;We further validated the dynamic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.;We further validated the dynamic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.;We further validated the dynamic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes.;We further validated the dynamic recruitment of TCF1 and Runx1 by ChIP-qPCR at the Tcra Enhancer, which is bound by similar levels of Ets1 in both DN and DP thymocytes. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene