About Enhancer
| Enhancer ID: | E_02_0849 |
| Species: | mouse |
| Position : | chr4:29115647-29140947   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 26537192 |
| Enhancer experiment: | PCR,Transgenic mice |
| Enhancer experiment description: | Transgenic LacZ reporter analysis, using overlapping EphA7 BACs, was used to narrow down the dorsal midline-specific enhancer, revealing the 25.3 kb genomic region as the enhancer candidate. Strikingly, this genomic DNA was located far downstream of the EphA7 transcription start site, +302.6 kb to +327.9 kb. |
About Target gene
| Target gene : | Epha7(Cek11,Ebk,Ehk3,Hek11,Mdk1),Epha7(Cek11,Ebk,Ehk3,Hek11,Mdk1),Epha7(Cek11,Ebk,Ehk3,Hek11,Mdk1) |
| Strong evidence: | -- |
| Less strong evidence: | PCR,Transgenic mice |
| Target gene experiment description: | Further enhancer mapping, using comparative genomic analysis and transgenic methods, showed that the 187 bp genomic DNA alone, approximately 305 kb downstream of the EphA7 transcription start site, was sufficient to act as the dorsal midline-specific enhancer of EphA7.;Further enhancer mapping, using comparative genomic analysis and transgenic methods, showed that the 187 bp genomic DNA alone, approximately 305 kb downstream of the EphA7 transcription start site, was sufficient to act as the dorsal midline-specific enhancer of EphA7.;Further enhancer mapping, using comparative genomic analysis and transgenic methods, showed that the 187 bp genomic DNA alone, approximately 305 kb downstream of the EphA7 transcription start site, was sufficient to act as the dorsal midline-specific enhancer of EphA7. |
About TF
| TF name : | Sbe3(ECR3)Sbe3(ECR3)Sbe3(ECR3) |
| TF experiment: | PCR |
| TF experiment description: | To investigate whether the conserved transcription factor binding sites are functionally important for the 187 bp ECR3 enhancer activity, three putative transcription factor binding sites were selected for deletion. The deleted ECR3 DNA was examined for its effect on the enhancer activity by creating transgenic mouse embryos (Figs. 4A and 4B).;To investigate whether the conserved transcription factor binding sites are functionally important for the 187 bp ECR3 enhancer activity, three putative transcription factor binding sites were selected for deletion. The deleted ECR3 DNA was examined for its effect on the enhancer activity by creating transgenic mouse embryos (Figs. 4A and 4B).;To investigate whether the conserved transcription factor binding sites are functionally important for the 187 bp ECR3 enhancer activity, three putative transcription factor binding sites were selected for deletion. The deleted ECR3 DNA was examined for its effect on the enhancer activity by creating transgenic mouse embryos (Figs. 4A and 4B). |
About Function
| Enhancer function : | The 187 bp dorsal midline-specific enhancer is critically regulated by homeobox transcription factors during the development of the dien- and mesencephalon. |
| Enhancer function experiment: | PCR |
| Enhancer function experiment description: |
Importantly, we found that transgenic embryos carrying ECR3, but not ECR1 or 2, displayed LacZ expression in the dorsal midline of the dien- and mesencephalon |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene