| Target gene experiment description: |
This locus has a well-character- ized Enhancer residing 114 kb upstream of the transcrip- tion start site (TSS),and its coding region encompasses ?82 kb,thus lending itself to finely space-and time-re- solved 3C analysis (Jing et al. 2008) No- tably,additional dynamic contacts of the Enhancer with the coding region were observed at the positions of elon- gating RNAPII. Similar dynamic Enhancer gene body con- tacts were identified at the CD47 locus.CD47 gene contacts with its distal enhancer at ?80 kb dur_x0002_ing synchronized transcriptional elongation. (A) Map of the CD47 lo_x0002_cus. Blue bars indicate fragments of BglII, and red bars indicate the regions used for ChIP and RT-qPCR.;This locus has a well-character- ized Enhancer residing 114 kb upstream of the transcrip- tion start site (TSS),and its coding region encompasses ?82 kb,thus lending itself to finely space-and time-re- solved 3C analysis (Jing et al. 2008) No- tably,additional dynamic contacts of the Enhancer with the coding region were observed at the positions of elon- gating RNAPII. Similar dynamic Enhancer gene body con- tacts were identified at the CD47 locus.CD47 gene contacts with its distal enhancer at ?80 kb dur_x0002_ing synchronized transcriptional elongation. (A) Map of the CD47 lo_x0002_cus. Blue bars indicate fragments of BglII, and red bars indicate the regions used for ChIP and RT-qPCR.;This locus has a well-character- ized Enhancer residing 114 kb upstream of the transcrip- tion start site (TSS),and its coding region encompasses ?82 kb,thus lending itself to finely space-and time-re- solved 3C analysis (Jing et al. 2008) No- tably,additional dynamic contacts of the Enhancer with the coding region were observed at the positions of elon- gating RNAPII. Similar dynamic Enhancer gene body con- tacts were identified at the CD47 locus.CD47 gene contacts with its distal enhancer at ?80 kb dur_x0002_ing synchronized transcriptional elongation. (A) Map of the CD47 lo_x0002_cus. Blue bars indicate fragments of BglII, and red bars indicate the regions used for ChIP and RT-qPCR.;This locus has a well-character- ized Enhancer residing 114 kb upstream of the transcrip- tion start site (TSS),and its coding region encompasses ?82 kb,thus lending itself to finely space-and time-re- solved 3C analysis (Jing et al. 2008) No- tably,additional dynamic contacts of the Enhancer with the coding region were observed at the positions of elon- gating RNAPII. Similar dynamic Enhancer gene body con- tacts were identified at the CD47 locus.CD47 gene contacts with its distal enhancer at ?80 kb dur_x0002_ing synchronized transcriptional elongation. (A) Map of the CD47 lo_x0002_cus. Blue bars indicate fragments of BglII, and red bars indicate the regions used for ChIP and RT-qPCR. |
