| Target gene experiment description: |
Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C).;Multiple promoter-interacting elements were detected in the Mef2c lo_x0002_cus, of which some showed a lower signal in Ldb1-deficientEBs (Figure 5B). Importantly, we observed interactions of the AHF enhancer with the promoter area and with the 30 end of the Mef2c gene, which were decreased in Ldb1-deficient EBs(Figure 5B). 3C-qPCR experiments revealed a similar long-range interaction pattern in WT versus Ldb1-/-EBs during the course of cardiac differentiation and in dissected SHF and hearts of E810.5 embryos, confirming the 3C-seq results (Figure 5C). |
