| Target gene experiment description: |
We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1.;We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1.;We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1.;We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1.;We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1.;We hypothesized the existence of an insulator-type element that prevents the spreadof DNA methylation within the1.8 k segment,and actually identified a 242- and a 185- fragments that were located adjacent to eachother and showed insulatorand Enhancer activities,respectively,in reporter assays. We designated these genomicregions as the Ccnb1ip1 insulator and the Ccnb1ip1Enhancer. The Ccnb1ip1 insulator showed Enhancer-blocking activity in the Luciferaseiferase assays andbarrieractivity in the colony formation assays. Further examination of the Ccnb1ip1locus in other mammalian speciesrevealed that the insulator and Enhancerare High throughput lyconserve damonga wide variety of species,and are located immediately upstream of the transcriptional start site of Ccnb1ip1. |
