About Enhancer
| Enhancer ID: |
E_02_0875 |
| Species: |
human |
| Position :
|
chr8:83287848-83288068
  |
| Biosample name: |
|
| Experiment class :
|
Low throughput |
| Enhancer type: |
Enhancer |
| Disease: |
-- |
| Pubmed ID: |
25644605
|
| Enhancer experiment: |
ChIP,RT-PCR |
| Enhancer experiment description: |
we hypothesized that PRDM16 might be recruited to the enhancer region of the Ucp1 gene through the interaction with PGC-1a. This possibility was tested in an immobilized template assay with purified proteins and a beadimmobilized DNA fragment spanning the 220-bp Ucp1 enhancer. |
About Target gene
| Target gene : |
Ucp1(AI385626,Slc25a7,Ucp) |
| Strong evidence: |
-- |
| Less strong evidence: |
ChIP,RT-PCR |
| Target gene experiment description: |
Overall, these results indicate that the ZF1 domain is required for the recruitment of PRDM16 to the Ucp1 enhancer through MED1 and PGC-1a. |
About TF
| TF name : |
Prdm16(5730557K01Rik,csp1,mel1) |
| TF experiment: |
ChIP |
| TF experiment description: |
To determine whether PRDM16 is recruited to the Enhancer following Ucp1 induction, we treated Med1 +/+MEFs that stably express PPARg and PRDM16 with forskolin and performed a chromatin immunoprecipitation (ChIP) assay with an antibody specific for PRDM16. |
About Function
| Enhancer function : |
-- |
| Enhancer function experiment: |
-- |
Enhancer function
experiment description: |
-- |
About SNP
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene
Expression of target genes for the
enhancer
Enhancer associated SNPs