About Enhancer
| Enhancer ID: | E_02_0883 |
| Species: | mouse |
| Position : | chr1:133303739-133325138   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 25486239 |
| Enhancer experiment: | DNase-seq,Immunofluorescence,Luciferase Reporter Assay,PCR,3C |
| Enhancer experiment description: | Using open Chromatin mapping by DNase-seq in HTE cell,the core of DHS-44 was localized to 600 at hg 19,Chr7:117075400-117076000.This region was marked by enrichment of H3K27Ac in 16HBE14o-cell,consistent with DHS-44 encompassing an active Enhancer element. |
About Target gene
| Target gene : | Kiss1(kisspeptin,metastatin) |
| Strong evidence: | 3C |
| Less strong evidence: | Transgenic mice |
| Target gene experiment description: | The present study aimed to determine ARC-specific enhancer(s) of the Kiss1 gene by an in vivo reporter assay.The 3C assay was performed to detect interactions between the Kiss1 promoter region and the 5 -upstream region, the latter of which was identified as an ARCspecific enhancer in the present study |
About TF
| TF name : | Eral1(2610524P08Rik,9130407C09Rik,AU019798,Era,M-ERA,MERA-S,MERA-W) |
| TF experiment: | ChIP |
| TF experiment description: | The binding of ERa in the 5' upstream region (Figure 4) was determined by a ChIP assay with ERa antibody. |
About Function
| Enhancer function : | The 5'-upstream region of Kiss1 locus functions as an enhancer for ARC Kiss1 gene expression in mice. |
| Enhancer function experiment: | 3C |
| Enhancer function experiment description: |
In addition,the current 3C and ER ChIP assays suggest that the Chromatin loop formation between the ARC-specific Enhancer and the promoter region of Kiss1 gene and unoccupied ER recruitment to the putative Enhancer region are involved in Kiss1 gene expression in the ARC |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene