About Enhancer
| Enhancer ID: | E_02_0885 |
| Species: | mouse |
| Position : | chr2:167687771-167689503   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 25453760 |
| Enhancer experiment: | ChIP-seq,ATAC-seq |
| Enhancer experiment description: | analysis of active enhancers (H3K27ac) revealed the same relationship between H3K27ac intensity and cell type specific genes. ChIP-Seq of in vivo splenic pDCs showed similar patterns to our in vitro pDC model with respect to H3K4me1 and H3K27ac in enhancer regions associated to pDC and moDC specific genes |
About Target gene
| Target gene : | Cebpb(C/EBPbeta,CRP2,IL-6DBP,LAP,LIP,NF-IL6,NF-M,Nfil6) |
| Strong evidence: | -- |
| Less strong evidence: | qPCR,RNA-seq,Western blot |
| Target gene experiment description: | Taken together,our results suggest that Irf8 and Cebpb form a double negative feedback loop and a positive self-auto-regulatory loop.Such a composite self-reinforcing negative feedback loop confers bi-stability,leading to either expression of one TF or the other;as a consequence, the entire epigenetic landscape is directed towards either a pDC or moDC Enhancer state. |
About TF
| TF name : | Tcf4(E2-2,FECD3,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4,bHLHb19)Spib(Spi-B)Bcl11a |
| TF experiment: | ATAC-seq,ChIP-seq |
| TF experiment description: | To better focus on open chromatin accessible to TF binding within these Enhancers, we narrowed down our search to overlapping peak of ATAC-seq signal. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Tcf4 | AndrogenReceptor | netpath | 167 |
| Tcf4 | CDO in myogenesis | reactome | 29 |
| Tcf4 | Coregulation of Androgen receptor activity | pid | 61 |
| Tcf4 | Formation of the beta-catenin:TCF transactivating complex | reactome | 88 |
| Tcf4 | ID | netpath | 38 |
| Tcf4 | Regulation of nuclear beta catenin signaling and target gene transcription | pid | 80 |
| Tcf4 | TGF_beta_Receptor | netpath | 220 |
| Tcf4 | Wnt | netpath | 118 |
| Tcf4 | Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 | wikipathways | 41 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene