About Enhancer
| Enhancer ID: | E_02_0887 |
| Species: | mouse |
| Position : | chr15:63254341-63256341   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Acute lymphoblastic leukemia |
| Pubmed ID: | 25369933 |
| Enhancer experiment: | ChIP-seq,EMSA,3C |
| Enhancer experiment description: | Notch1 is depleted from the NDME site by short-term gammasecretase inhibitor (GSI) treatment, and loading and unloading of Notch1 is associated with rapid changes in H3K27ac, features that characterize genomic Notch1 binding sites that dynamically regulate gene expression.Notch1 also bound the NDME in primary murine T-ALL cells and in DN3 thymocytes, a stage of T-cell development marked by high levels of Notch1 activation |
About Target gene
| Target gene : | Myc(MRTLC,bHLHe39,c-Myc),Myc(MRTLC,bHLHe39,c-Myc) |
| Strong evidence: | 3C |
| Less strong evidence: | ChIP,qRT-PCR,Luciferase Reporter Assay |
| Target gene experiment description: | Schematic of the Luciferasegene constructs including the NDME WT and mutant sequences.To test for chromatin looping between the NDME and the Myc promoter, chromatin conformation capture (3C) assays were carried out in T6E cells.;Schematic of the Luciferasegene constructs including the NDME WT and mutant sequences.To test for chromatin looping between the NDME and the Myc promoter, chromatin conformation capture (3C) assays were carried out in T6E cells. |
About TF
| TF name : | Notch(Notch)Notch(Notch) |
| TF experiment: | ChIP-seq,3C |
| TF experiment description: | Analysis of the ChIP-seq data revealed an RBPJ/Notch1 binding site associated with high levels of H3K4me1 marks located 1.43 Mb 3? of the Myc promoter (Fig. 4A) that shares sequence homology with the murine NDME.;Analysis of the ChIP-seq data revealed an RBPJ/Notch1 binding site associated with high levels of H3K4me1 marks located 1.43 Mb 3? of the Myc promoter (Fig. 4A) that shares sequence homology with the murine NDME. |
About Function
| Enhancer function : | Altered long-range enhancer activity can mediate resistance to targeted therapies and provide a mechanistic rationale for combined targeting of Notch and Brd4 in leukemia. |
| Enhancer function experiment: | 3C |
| Enhancer function experiment description: |
To determine whether Notch signaling was required to maintain the interaction between the Myc promoter and the NDME,3C analysis was performed in T6E cells cultured in the presence or absence of GSI. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene