About Enhancer
| Enhancer ID: | E_02_0888 |
| Species: | mouse |
| Position : | chr10:34385685-34385834   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Skeletal diseases |
| Pubmed ID: | 25321476 |
| Enhancer experiment: | Transgenic mice,ChIP,qRT-PCR,EMSA |
| Enhancer experiment description: | These results show that multiple copies of the enhancer (150-bp Col10a1 distal promoter) mediates higher level and cellspecific reporter activity, whereas the 10-kb Col10a1 promoter/intronic element contains nonspecific regulatory elements,in addition to the 150-bp enhancer. |
About Target gene
| Target gene : | Col10a1(Col10,Col10a-1) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,qRT-PCR,EMSA |
| Target gene experiment description: | Top panel displays Col10a1 gene structure and the 10 -kb promoter and intronic element.Positions of the 150-bp (?4296 to ?4147 bp) Col10a1 cis-Enhancer (purple bar) and its 330-bp (?220 to +110 bp) basal promoter (red bar) are shown. |
About TF
| TF name : | Runx2(AML3,Cbf,Cbfa-1,Cbfa1,LS3,Osf2,PEBP2aA,Pebp2a1,Pebpa2a) |
| TF experiment: | qRT-PCR,EMSA |
| TF experiment description: | The qRT-PCR was performed to examine the mRNA transcript level of marker genes Col10a1 and Runx2 in MCT cells. As illustrated, both Col10a1 and Runx2 are significantly upregulated in hypertrophic MCT cells compared with that in proliferative MCT cells. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Runx2 | AndrogenReceptor | netpath | 167 |
| Runx2 | FGF signaling pathway | pid | 55 |
| Runx2 | Leptin | netpath | 98 |
| Runx2 | Notch-mediated HES/HEY network | pid | 48 |
| Runx2 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Runx2 | Regulation of retinoblastoma protein | pid | 67 |
| Runx2 | TGF_beta_Receptor | netpath | 220 |
| Runx2 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| Runx2 | Hs_MAPK_Signaling_Pathway_WP382_79951 | wikipathways | 58 |
| Runx2 | Hs_Endochondral_Ossification_WP474_87977 | wikipathways | 43 |
| Runx2 | Hs_Role_of_Osx_and_miRNAs_in_tooth_development_WP3971_91525 | wikipathways | 9 |
| Runx2 | Hs_Bone_Morphogenic_Protein_(BMP)_Signalling_and_Regulation_WP1425_86082 | wikipathways | 6 |
| Runx2 | Hs_Focal_Adhesion-PI3K-Akt-mTOR-signaling_pathway_WP3932_91101 | wikipathways | 8 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene