About Enhancer
| Enhancer ID: | E_02_0889 |
| Species: | human |
| Position : | chr7:94002773-94005073   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Systemic sclerosis |
| Pubmed ID: | 25303440 |
| Enhancer experiment: | Luciferase Reporter Assay |
| Enhancer experiment description: | TGF? is a potent inducer of the human procollagen type I ?2 chain gene ( COL1A2). We have recently described in detail the in vivo mechanism underlying the transcriptional control of COL1A2, through a complex interaction between its distal enhancer and proximal promoter, in response to TGF?. Reporter gene constructs containing the wild-type human enhancer region and the heterologous thymidine kinase (TK) promoter, lacking a Smad-dependent T RE (21.1/18.8pLAC-TK), were used to assess the T?RE in the FUE region. The results indicated that the COL1A2-FUE was activated by TGF? in both normal and scleroderma fibroblasts. |
About Target gene
| Target gene : | COL1A2(EDSARTH2,EDSCV,OI4) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,EMSA,ChIP,siRNA |
| Target gene experiment description: | Gene activation was determined by assessing the interaction of transcription factors with the COL1A2 Enhancer using transient transfection of reporter gene constructs,electrophoretic mobility shift assays,chromatin immunoprecipitation analysis,and RNA interference involving knockdown of individual AP-1 family members. |
About TF
| TF name : | JUNB(AP-1)JUND(AP-1) |
| TF experiment: | EMSA,ChIP |
| TF experiment description: | Supershift assays using specific antibodies (Figure 2B) revealed that normal nuclear extracts bound c-Jun and, to a lesser extent, JunD. In SSc, c-Jun binding was greatly reduced, and JunB, which was previously absent in normal nuclear extracts, accounted for 2 of the shifted bands. JunD binding was more pronounced in SSc extracts (Figure 2B). These gel-shift findings suggest that in SSc dermal fibroblasts or in normal nuclea extracts treated with TGF , JunB binding replaces c-Jun binding on the AP-1 site in the upstream enhancer.These results were confirmed using ChIP assays,which demonstrated the association of the F2 region in the FUE with the proximal promoter and the transcriptional machinery. |
About Function
| Enhancer function : | Binding of JunB to the COL1A2 enhancer was observed, with its coalescence directed by activation of gene transcription through the proximal promoter. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
RNA polymeraseII also coprecipitated with the F2 Enhancer sequence,despite not being able to bind the Enhancer directly,thereby demonstrating the interaction between the transcriptional machinery in the promoter with the factors bound to the F2 region. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene