About Enhancer

Enhancer ID: E_02_0890
Species: human
Position : chr11:102006458-102008458
Biosample name:
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  25271055
Enhancer experiment: Luciferase Reporter Assay,ChIP
Enhancer experiment description: Three lines of evidence support a direct role for HDAC5 in regulating MEF2C activity at the +45 kB SOST enhancer over the course of osteocyte differentiation. First, HDAC5 shRNA causes increased activity of this element, but not of the proximal SOST promoter, in luciferase assays. MEF2C shRNA reduces the activity of this enhancer. Second, HDAC5 overexpression dose-dependently inhibits the activity of a MEF2-driven reporter from the desmin and the SOST9 (+45kB) enhancer. Third, endogenous HDAC5 association with this region in control (but not HDAC5 shRNA) Ocy454 cells can be detected by ChIP.

About Target gene

Target gene : Sost(5430411E23Rik),Sost(5430411E23Rik),Sost(5430411E23Rik)
Strong evidence: --
Less strong evidence: ChIP,qRT-PCR,Western blot
Target gene experiment description: Using Chromatin immunoprecipitation,we have mapped endogenous MEF2C binding in the SOST gene to a distal intergenic Enhancer 45 downstream from the transcription start site.;Using Chromatin immunoprecipitation,we have mapped endogenous MEF2C binding in the SOST gene to a distal intergenic Enhancer 45 downstream from the transcription start site.;Using Chromatin immunoprecipitation,we have mapped endogenous MEF2C binding in the SOST gene to a distal intergenic Enhancer 45 downstream from the transcription start site.

About TF

TF name : Mef2c(5430401D19Rik,9930028G15Rik,AV011172,Mef2)Mef2c(5430401D19Rik,9930028G15Rik,AV011172,Mef2)Mef2c(5430401D19Rik,9930028G15Rik,AV011172,Mef2)
TF experiment: ChIP,PCR
TF experiment description: We performed Chromatin immunoprecipitation (ChIP) to determine MEF2C occupancy in Ocy454 cell cultured at 37C for 14 days (a time point in which SOST expression is High throughput).;We performed Chromatin immunoprecipitation (ChIP) to determine MEF2C occupancy in Ocy454 cell cultured at 37C for 14 days (a time point in which SOST expression is High throughput).;We performed Chromatin immunoprecipitation (ChIP) to determine MEF2C occupancy in Ocy454 cell cultured at 37C for 14 days (a time point in which SOST expression is High throughput).

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
Mef2c CDO in myogenesis reactome 29
Mef2c Circadian Clock reactome 49
Mef2c ErbB1 downstream signaling pid 105
Mef2c ERK/MAPK targets reactome 17
Mef2c Notch netpath 76
Mef2c Oxidative stress response panther 23
Mef2c p38 MAPK pathway panther 31
Mef2c Regulation of nuclear SMAD2/3 signaling pid 82
Mef2c Regulation of retinoblastoma protein pid 67
Mef2c Signaling events mediated by HDAC Class II pid 58
Mef2c Signaling mediated by p38-alpha and p38-beta pid 35
Mef2c TGF_beta_Receptor netpath 220
Mef2c Transcriptional activation of mitochondrial biogenesis reactome 43
Mef2c Trk receptor signaling mediated by the MAPK pathway pid 34
Mef2c MAPK signaling pathway kegg 264
Mef2c Hs_Endochondral_Ossification_WP474_87977 wikipathways 43
Mef2c Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
Mef2c Hs_Heart_Development_WP1591_90186 wikipathways 28
Mef2c Hs_Oxidation_by_Cytochrome_P450_WP43_84102 wikipathways 14
Mef2c Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67
Mef2c CDO in myogenesis reactome 29
Mef2c Circadian Clock reactome 49
Mef2c ErbB1 downstream signaling pid 105
Mef2c ERK/MAPK targets reactome 17
Mef2c Notch netpath 76
Mef2c Oxidative stress response panther 23
Mef2c p38 MAPK pathway panther 31
Mef2c Regulation of nuclear SMAD2/3 signaling pid 82
Mef2c Regulation of retinoblastoma protein pid 67
Mef2c Signaling events mediated by HDAC Class II pid 58
Mef2c Signaling mediated by p38-alpha and p38-beta pid 35
Mef2c TGF_beta_Receptor netpath 220
Mef2c Transcriptional activation of mitochondrial biogenesis reactome 43
Mef2c Trk receptor signaling mediated by the MAPK pathway pid 34
Mef2c MAPK signaling pathway kegg 264
Mef2c Hs_Endochondral_Ossification_WP474_87977 wikipathways 43
Mef2c Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
Mef2c Hs_Heart_Development_WP1591_90186 wikipathways 28
Mef2c Hs_Oxidation_by_Cytochrome_P450_WP43_84102 wikipathways 14
Mef2c Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67
Mef2c CDO in myogenesis reactome 29
Mef2c Circadian Clock reactome 49
Mef2c ErbB1 downstream signaling pid 105
Mef2c ERK/MAPK targets reactome 17
Mef2c Notch netpath 76
Mef2c Oxidative stress response panther 23
Mef2c p38 MAPK pathway panther 31
Mef2c Regulation of nuclear SMAD2/3 signaling pid 82
Mef2c Regulation of retinoblastoma protein pid 67
Mef2c Signaling events mediated by HDAC Class II pid 58
Mef2c Signaling mediated by p38-alpha and p38-beta pid 35
Mef2c TGF_beta_Receptor netpath 220
Mef2c Transcriptional activation of mitochondrial biogenesis reactome 43
Mef2c Trk receptor signaling mediated by the MAPK pathway pid 34
Mef2c MAPK signaling pathway kegg 264
Mef2c Hs_Endochondral_Ossification_WP474_87977 wikipathways 43
Mef2c Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 wikipathways 153
Mef2c Hs_Heart_Development_WP1591_90186 wikipathways 28
Mef2c Hs_Oxidation_by_Cytochrome_P450_WP43_84102 wikipathways 14
Mef2c Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs