About Enhancer
| Enhancer ID: | E_02_0892 |
| Species: | mouse |
| Position : | chr1:182851627-182852853   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 25263596 |
| Enhancer experiment: | ChIP-seq,ChIP |
| Enhancer experiment description: | We therefore hypothesized that loss of Tet2 could lead to reduced oxidation and increased 5mC at enhancers. Indeed, hypermethylated DMRs (hyper-DMRs) in Tet2?/? cells exhibit several hallmarks of enhancers including: evolutionary sequence conservation,enrichment for enhancer chromatin marks H3K4me1 and H3K27ac, and significant overlap with co-activator p300 binding sites (o/e = 5.9, p < 1E-200), DNase I hypersensitive sites(o/e = 4.5, p < 1E-200), and predicted enhancers(o/e = 7.1, p < 1E-200) |
About Target gene
| Target gene : | Lefty1(LEFTB,LEFTYB) |
| Strong evidence: | -- |
| Less strong evidence: | PCR,Transgenic mice |
| Target gene experiment description: | Indeed,an Enhancer that physically interacts with the developmental gene Left-Right Determination Factor 1 (Lefty1) is hypermethylated and hypoacetylated in Tet6?/? cells,potentially explaining the significantly decreased expression of this gene. |
About TF
| TF name : | Oct4Sox4(AA682046,Sox-4)Nanog(NANOG) |
| TF experiment: | ChIP-seq |
| TF experiment description: | Weaker occupancy of the ES-cell core transcription factors (TFs) OCT4,SOX6,and NANOG than those that do not change DNA methylation status. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox4 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox4 | IL5 | netpath | 59 |
| Nanog | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Nanog | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Nanog | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene