About Enhancer
| Enhancer ID: | E_02_0894 |
| Species: | mouse |
| Position : | chr9:99131669-99132862   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Heart injuries |
| Pubmed ID: | 25249570 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP-seq |
| Enhancer experiment description: | To measure the transcriptional activity of the YAP-bound region of Pik3cb, which we refer to as the Pik3cb enhancer, we cloned a 552 bp genomic DNA fragment containing the conserved element and potential TEAD binding sites into a minimal promoter luciferase reporter construct. Co-transfection with Yap in NRVMs showed that Yap stimulates activity of the enhancer by ~5-fold. |
About Target gene
| Target gene : | Pik3cb(1110001J02Rik,AI447572,p110beta) |
| Strong evidence: | -- |
| Less strong evidence: | qRT-PCR,ChIP-qPCR |
| Target gene experiment description: | To measure the transcriptional activity of the YAP-bound region of Pik3cb, which we refer to as the Pik3cb Enhancer, we cloned a 552 bp genomic DNA fragment containing the conserved element and potential TEAD binding sites into a minimal promoter luciferase reporter construct. |
About TF
| TF name : | Yap1(AI325207,Yap,Yap65,Yki,Yorkie) |
| TF experiment: | ChIP-seq,ChIP-qPCR,Luciferase Reporter Assay |
| TF experiment description: | The HL1 ChIP-seq data revealed a YAP-bound sequence residing in the first intron of Pik3cb (Fig. 2A). We validated YAP binding to the identified sequence by ChIP-qPCR,using a pair of primers spanning the YAP bound sequence and a control pair recognizing a sequence 1.3 kb away. |
About Function
| Enhancer function : | YAP and TEAD occupied a conserved Enhancer within the first intron of Pik3cb, and this Enhancer drove YAP_x0002_dependent reporter gene expression. |
| Enhancer function experiment: | ChIP-seq,ChIP-qPCR,Luciferase Reporter Assay |
| Enhancer function experiment description: |
To measure the transcriptional activity of the YAP-bound region of Pik3cb, which we refer to as the Pik3cb Enhancer, we cloned a 552 bp genomic DNA fragment containing the conserved element and potential TEAD binding sites into a minimal promoter luciferase reporter construct. Co-transfection with Yap in NRVMs showed that Yap stimulates activity of the Enhancer by ~5-fold. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Yap1 | ErbB4 signaling events | pid | 35 |
| Yap1 | Nuclear signaling by ERBB4 | reactome | 25 |
| Yap1 | p73 transcription factor network | pid | 80 |
| Yap1 | PPARA activates gene expression | reactome | 113 |
| Yap1 | Signaling by Hippo | reactome | 20 |
| Yap1 | TGF-beta receptor signaling | pid | 53 |
| Yap1 | TGF_beta_Receptor | netpath | 220 |
| Yap1 | Validated transcriptional targets of deltaNp63 isoforms | pid | 47 |
| Yap1 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| Yap1 | Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 | wikipathways | 50 |
| Yap1 | Hs_miR-509-3p_alteration_of_YAP1-ECM_axis_WP3967_91491 | wikipathways | 14 |
| Yap1 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| Yap1 | Hs_TGF-beta_Signaling_Pathway_WP366_90028 | wikipathways | 67 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene