About Enhancer
| Enhancer ID: | E_02_0896 |
| Species: | mouse |
| Position : | chr17:35502632-35504632   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 25223790 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,ChIP-qPCR |
| Enhancer experiment description: | We first investigated activation of the Oct4 enhancer by luciferase reporter assay 48 h after transfection of TALE-A and dCas9-A/gRNA in MEFs. These luciferase constructs contain the 2.4 kb region covering all three upstream regulatory elements of the Oct4 locus |
About Target gene
| Target gene : | Oct4 |
| Strong evidence: | CRISPR/Cas9 |
| Less strong evidence: | qRT-PCR,Luciferase Reporter Assay |
| Target gene experiment description: | The Oct4 luciferase assay reporter constructs carried the genomic DNA 2.4 kb upstream of the Oct4 transcrip_x0002_tion start site (TSS). The region encompasses the 1.7 kb distal and proximal Enhancers and the 0.2 kb promoter. |
About TF
| TF name : | Klf4(EZF,GKLF)Oct4Nanog(NANOG)Sox2(ANOP3,MCOPS3) |
| TF experiment: | ChIP-seq |
| TF experiment description: | To address this possibility, we reviewed the ChIP-seq information of several pluripotency transcription factors, including KLF4,OCT4,NANOG and SOX2 at the Nanog 5kb upstream Enhancer region(42) and found that the Site 2 (targeted by both TALE and dCas9) was surrounded by the predicted KLF4 and NANOG binding sites. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene