About Enhancer
| Enhancer ID: | E_02_0898 |
| Species: | human |
| Position : | chr8:130179643-130181490   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | T-cell acute lymphoblastic leukemia |
| Pubmed ID: | 25194570 |
| Enhancer experiment: | ChIP-seq,ChIP,Luciferase Reporter Assay,3C,PCR |
| Enhancer experiment description: | This highly conserved regulatory element, hereby named N-Me for NOTCH MYC enhancer, is located within a broad super-enhancer region +1.47 Mb from the MYC transcription initiating site,interacts with the MYC proximal promoter and induces orientation-independent MYC expression in reporter assays.Moreover, luciferase reporter assays showed strong, orientationindependent activation of reporter constructs containing this enhancer in association with a ?2.5 kb MYC proximal promoter21 in JURKAT T-ALL cells,which express high levels of constitutively active NOTCH1 protein 22, but not in Daudi and Raji B-cell lineage cells. |
About Target gene
| Target gene : | MYC(MRTLC,bHLHe39,c-Myc) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,PCR |
| Target gene experiment description: | Consistent with this hypothesis, chromatin configuration 3C analysis of the MYC locus demonstrated the association of this enhancer with proximal regulatory sequences in the MYC promoter.Moreover, histological analysis of multiple tissues including those in which NOTCH signaling plays important developmental roles such as breast epithelium, skin, and intestine showed no alterations (Supplementary Fig. 7) and RT-PCR analysis showed no effects of N_x0002_Me deletion in Myc expression. |
About TF
| TF name : | NOTCH1(AOS5,AOVD1,TAN1,hN1) |
| TF experiment: | ChIP,ChIP-seq |
| TF experiment description: | Interestingly, chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-seq) analysis of NOTCH1 chromatin binding sites in HPB-ALL T-ALL cells revealed a prominent 1 kb NOTCH1 peak in chromosome 8q24 located within the common 40 Kb segment duplicated in all these eight leukemia cases. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene