About Enhancer
| Enhancer ID: | E_02_0899 |
| Species: | mouse |
| Position : | chr17:31172998-31173656   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 25030696 |
| Enhancer experiment: | 3C,ChIP,RNA-seq,GRO-seq,Luciferase Reporter Assay |
| Enhancer experiment description: | Interestingly, primarily RXR-induced eRNA production could be detected on an enhancer assigned to Vegfa or Tgm2, while an enhancer of Abcg1 also showed robust LXR ligand activation, as expected. These data suggested that the eRNAs can be easily validated and show ligand induction similar to the regulated genes and therefore most likely are linked. |
About Target gene
| Target gene : | Abcg1(AW413978,Abc8,White) |
| Strong evidence: | 3C |
| Less strong evidence: | qRT-PCR |
| Target gene experiment description: | Box plot representation of the distribution of interaction frequency of Abcg1 B1 Enhancer (chr17: 31,172,99831,173,656) and Vegfa B1 Enhancer (chr17: 45,890,06045,890,829) determined by 3C-seq. |
About TF
| TF name : | Rxra(9530071D11Rik,Nr2b1,RXRalpha1) |
| TF experiment: | RNA-seq,ChIP-seq,GRO-seq,3C-seq |
| TF experiment description: | In our studies, we tried to solve these issues by combining RNA-seq, ChIP-seq, GRO-seq (global run-on sequencing), and 3C-seq (chromosome conformation capture [3C] combined with sequencing) in a highly integrated way to unravel the mechanism of RXR-induced transcriptional events in mouse bone marrow-derived macrophages (BMDMs) and, as the result of the process,discovered and validated a novel biological activity promoted by the receptor. |
About Function
| Enhancer function : | A set of angiogenic genes, including Vegfa, has liganded RXR-controlled enhancers and provides the macrophage with a novel inducible program |
| Enhancer function experiment: | 3C,3C-seq |
| Enhancer function experiment description: |
Using chromosome conformation capture (3C) and 3C combined with sequencing (3C-seq), and we show that selected long-range Enhancers communicate with promoters via stable or RXR-induced loops and that some of the Enhancers interact with each other, forming an interchromosomal network. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene