About Enhancer
| Enhancer ID: | E_02_0915 |
| Species: | mouse |
| Position : | chr4:147962887-147964479   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | Heart failure |
| Pubmed ID: | 24391132 |
| Enhancer experiment: | RT-PCR,3C,ChIP,Luciferase Reporter Assay |
| Enhancer experiment description: | Among the 11 CRs identified, only CR9 coincided with the binding sites of RNA polymerase II and p300, and overlapped with the gene areas modified by H3K4me1, and filled all criteria for the enhancer. |
About Target gene
| Target gene : | Nppb(AA408272,BNF,BNP),Nppb(AA408272,BNF,BNP) |
| Strong evidence: | 3C |
| Less strong evidence: | RT-PCR,Transgenic mice |
| Target gene experiment description: | The Enhancer is a 650-bp fragment within 50 kb of the Nppa and Nppb loci.To identify the organs in which CR9 functioned as a stress-responsive Enhancer, we examined the luciferase reporter expression in each organ by quantitative PCR.;The Enhancer is a 650-bp fragment within 50 kb of the Nppa and Nppb loci.To identify the organs in which CR9 functioned as a stress-responsive Enhancer, we examined the luciferase reporter expression in each organ by quantitative PCR. |
About TF
| TF name : | Nkx2-5(Csx,Nkx-2.5,Nkx2.5,tinman)Ctcf(MRD21)Nkx2-5(Csx,Nkx-2.5,Nkx2.5,tinman)Ctcf(MRD21) |
| TF experiment: | ChIP-seq |
| TF experiment description: | ChIP-seq data for H3K4me1, p300, and CTCF were obtained from an open database of the adult mouse heart. Some CRs coincided with the peaks for H3K4me1, RNA polymerase II, and the transcriptional coactivator protein p300.;ChIP-seq data for H3K4me1, p300, and CTCF were obtained from an open database of the adult mouse heart. Some CRs coincided with the peaks for H3K4me1, RNA polymerase II, and the transcriptional coactivator protein p300. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nkx2-5 | Physiological factors | reactome | 12 |
| Nkx2-5 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Nkx2-5 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| Nkx2-5 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| Ctcf | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| Ctcf | TGF_beta_Receptor | netpath | 220 |
| Nkx2-5 | Physiological factors | reactome | 12 |
| Nkx2-5 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Nkx2-5 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| Nkx2-5 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| Ctcf | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
| Ctcf | TGF_beta_Receptor | netpath | 220 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene