About Enhancer

Enhancer ID: E_02_0918
Species: human
Position : chr11:110716521-110832306
Biosample name:
Experiment class : Low+High throughput
Enhancer type: Super-Enhancer
Disease: Diffuse large b-cell lymphoma
Pubmed ID:  24332044
Enhancer experiment: ChIP-seq,ChIP-qPCR
Enhancer experiment description: As predicted, BRD4 load is asymmetrically distributed throughout the genome at enhancer sites. Completely unexpected is the magnitude by which BRD4 load varies among active enhancer regions (Figure 4E). Only a small subset of BRD-loaded enhancers, 285/18330(1.6%), account for 32% of all of the BRD4 enhancer binding in the cell.The BRD4-loaded enhancers in the Ly1 DLBCL cell line are considerably larger than typical enhancer elements, resembling the super-enhancers we recently described with Richard Young.

About Target gene

Target gene : POU2AF1(BOB1,OBF-1,OBF1,OCAB)
Strong evidence: --
Less strong evidence: ChIP-seq
Target gene experiment description: Notably, the top two gene loci with BRD4-loaded enhancers, POU2AF1 (which encodes the OCA-B transcriptional co-activator protein) and BCL6, and additional genes with disproportionally BRD4-loaded enhancers such as PAX5 and IRF8 (Figure 4E), are essential for B-cell fate determination and germinal center formation.

About TF

TF name : E2F1(E2F-1,RBAP1,RBBP3,RBP3)
TF experiment: ChIP-seq,ChIP-qPCR
TF experiment description: Then, we assessed the genome-wide localization of E2F1 and the representative BET protein, BRD4, also by ChIP-Seq using the respective antibodies. Rank-ordering of all transcriptionally active promoters based on H3K4me3 enrichment and RNA Pol II occupancy identifies pervasive binding of BRD4 and E2F1 to active promoter elements.

About Function

Enhancer function : These super-Enhancers prove particularly sensitive to bromodomain inhibition,explaining the selective effect of BET inhibitors on oncogenic and lineage-specific transcriptional circuits.
Enhancer function experiment: ChIP-seq,ChIP-qPCR
Enhancer function
experiment description:		 These super-enhancers prove particularly sensitive to bromodomain inhibition, explaining the selective effect of BET inhibitors on oncogenic and lineage-specific transcriptional circuits. Functional study of genes marked by super-enhancers identifies DLBCLs dependent on OCA-B and suggests a strategy for discovering unrecognized cancer dependencies.

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
E2F1 Activation of PUMA and translocation to mitochondria reactome 8
E2F1 Association of licensing factors with the pre-replicative complex reactome 15
E2F1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
E2F1 CDC6 association with the ORC:origin complex reactome 11
E2F1 Cyclin D associated events in G1 reactome 38
E2F1 Direct p53 effectors pid 141
E2F1 E2F mediated regulation of DNA replication reactome 17
E2F1 E2F transcription factor network pid 77
E2F1 G0 and Early G1 reactome 25
E2F1 G1/S-Specific Transcription reactome 17
E2F1 G2 Phase reactome 5
E2F1 IL2 signaling events mediated by PI3K pid 38
E2F1 Inhibition of replication initiation of damaged DNA by RB1/E2F1 reactome 12
E2F1 Notch-mediated HES/HEY network pid 48
E2F1 Oncogene Induced Senescence reactome 30
E2F1 Oxidative Stress Induced Senescence reactome 120
E2F1 p75(NTR)-mediated signaling pid 74
E2F1 Pre-NOTCH Transcription and Translation reactome 29
E2F1 Regulation of retinoblastoma protein pid 67
E2F1 Regulation of Telomerase pid 70
E2F1 TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest reactome 13
E2F1 Cell cycle kegg 124
E2F1 Pathways in cancer kegg 321
E2F1 Pancreatic cancer kegg 70
E2F1 Glioma kegg 65
E2F1 Prostate cancer kegg 85
E2F1 Melanoma kegg 69
E2F1 Bladder cancer kegg 38
E2F1 Chronic myeloid leukemia kegg 69
E2F1 Small cell lung cancer kegg 83
E2F1 Non-small cell lung cancer kegg 54
E2F1 Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
E2F1 Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
E2F1 Hs_miRNAs_involved_in_DNA_damage_response_WP1545_84697 wikipathways 30
E2F1 Hs_Endochondral_Ossification_WP474_87977 wikipathways 43
E2F1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
E2F1 Hs_Integrated_Cancer_Pathway_WP1971_82939 wikipathways 33
E2F1 Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 wikipathways 50
E2F1 Hs_Bladder_Cancer_WP2828_89143 wikipathways 17
E2F1 Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520 wikipathways 16
E2F1 Hs_Aryl_Hydrocarbon_Receptor_WP2586_91687 wikipathways 16
E2F1 Hs_Fatty_Acid_Beta_Oxidation_WP143_79783 wikipathways 14
E2F1 Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs