About Enhancer
| Enhancer ID: | E_02_0919 |
| Species: | human |
| Position : | chr17:72860066-72860183   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 24321386 |
| Enhancer experiment: | ChIP,Luciferase Reporter Assay,PCR,EMSA |
| Enhancer experiment description: | Next, histone modifi- cation in the SF-1-induced cells was examined via ChIP assays, using antibodies against two active enhancer markers, histone H3K27ac and H3K4me2, to determine alterations of chromatin state within the region. The results clearly showed that the signals of these active enhancer markers around the SF-1 binding region increased along with the transduction of SF-1 (Fig. 1D and E), suggesting that the transduction of SF-1 led to the active state of chromatin within the FDXR intronic region. |
About Target gene
| Target gene : | FDXR(ADR,ADXR,ANOA) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,Luciferase Reporter Assay,PCR |
| Target gene experiment description: | Genome-wide analysis of differentiated cells, using a combination of DNA microarray and promoter tiling array analyses, showed that FDXR is a novel SF-1 target gene. In this study,the transcriptional regulatory mechanism of FDXR was examined in steroidogenic cells. |
About TF
| TF name : | NR5A1(AD4BP,ELP,FTZ1,FTZF1,POF7,SF-1,SF1,SPGF8,SRXX4,SRXY3,hSF-1) |
| TF experiment: | ChIP,qPCR |
| TF experiment description: | Closed and open squares represent Flag-tagged SF-1 (FlagSF-1)- and GFP (Control)-transduced MSCs, respectively; real-time PCR analysis of immunoprecipitated chromatin performed using primers for indicated genomic region; and expressed as a percentage of input. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| NR5A1 | Nuclear Receptor transcription pathway | reactome | 51 |
| NR5A1 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene