About Enhancer
| Enhancer ID: | E_02_0921 |
| Species: | mouse |
| Position : | chr2:119321593-119322529   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 24257627 |
| Enhancer experiment: | ChIP,PCR,EMSA |
| Enhancer experiment description: | In addition, we carried out chromatin immunoprecipitation (ChIP) assays to show that both Ascl1 and Neurog1 were able to occupy in vivo the critical E-box region of the CR1 Enhancer in cell culture and mouse embryonic neural tubes, whereas no enrichment was shown for control DNA in Dll4 3? UTR. |
About Target gene
| Target gene : | Dll4(Delta4) |
| Strong evidence: | -- |
| Less strong evidence: | EMSA |
| Target gene experiment description: | We performed an electrophoretic mobility shift assay (EMSA) to test whether Ascl1 and Neurog proteins were able to directly bind to the minimal enhancer region using an oligo probe containing the critical E-box (Probe 2) and a control upstream probe that lacks an E-box (Probe 1) |
About TF
| TF name : | Foxn4(Foxn4)Ascl1(AI225900,ASH1,Mash1,bHLHa46) |
| TF experiment: | EMSA,ChIP |
| TF experiment description: | We performed an electrophoretic mobility shift assay (EMSA) to test whether Ascl1 and Neurog proteins were able to directly bind to the minimal Enhancer region using an oligo probe containing the critical E-box (Probe 2) and a control upstream probe that lacks an E-box. |
About Function
| Enhancer function : | Foxn4, Ascl1 and Neurog1 were able to activate gene expression through an evolutionarily conserved Dll4 Enhancer CR1 found to be active in the retina. |
| Enhancer function experiment: | ChIP,EMSA |
| Enhancer function experiment description: |
In addition, we carried out chromatin immunoprecipitation (ChIP) assays to show that both Ascl1 and Neurog1 were able to occupy in vivo the critical E-box region of the CR1 Enhancer in cell culture and mouse embryonic neural tubes, whereas no enrichment was shown for control DNA in Dll4 3? UTR.Thus, Ascl1 and Neurog factors may bind to the same E-box in the Dll4 Enhancer but have differential effects on Dll4 gene expression. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Ascl1 | Notch-mediated HES/HEY network | pid | 48 |
| Ascl1 | Notch | netpath | 76 |
| Ascl1 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| Ascl1 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene