About Enhancer
| Enhancer ID: | E_02_0926 |
| Species: | mouse |
| Position : | chr5:107680655-107682655   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 24014243 |
| Enhancer experiment: | ChIP,PCR,Luciferase Reporter Assay |
| Enhancer experiment description: | To investigate whether Mysm1 regulates Gfi1 expression by binding to the regulatory elements of Gfi1 in HSC, we performed chromatin immunoprecipitation (ChIP) assays along the Gfi1 regulatory elements. A panel of PCR primers to encompass the 23.4-kb promoter and 235-kb enhancer of the Gfi1 locus was designed (Figure 5A). |
About Target gene
| Target gene : | Gfi1(GFI-1,GFI1A,SCN2,ZNF163) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,PCR |
| Target gene experiment description: | To investigate whether Mysm1 regulates Gfi1 expression by binding to the regulatory elements of Gfi1 in HSC, we performed chromatin immunoprecipitation (ChIP) assays along the Gfi1 regulatory elements. A panel of PCR primers to encompass the 23.4-kb promoter and 235-kb enhancer of the Gfi1 locus was designed (Figure 5A). |
About TF
| TF name : | Gata2(DCML,IMD21,MONOMAC,NFE1B)Runx1(AML1,AML1-EVI-1,AMLCR1,CBF2alpha,CBFA2,EVI-1,PEBP2aB,PEBP2alpha) |
| TF experiment: | Luciferase Reporter Assay,ChIP,PCR |
| TF experiment description: | Mechanistic studies revealed that Mysm1 modulates histone modifications and directs the recruitment of key transcriptional factors such as Gata2 and Runx1 to the Gfi1 locus in HSCs. We found that Mysm1 directly associates with the Gfi1 Enhancer element and promotes its transcription through Gata2 and Runx1 transactivation. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Gata2 | AP-1 transcription factor network | pid | 71 |
| Gata2 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| Gata2 | HIF-1-alpha transcription factor network | pid | 67 |
| Gata2 | IL3 | netpath | 84 |
| Gata2 | Regulation of Androgen receptor activity | pid | 54 |
| Gata2 | Signaling events mediated by HDAC Class I | pid | 89 |
| Gata2 | Signaling events mediated by HDAC Class II | pid | 58 |
| Gata2 | Hs_Apoptosis_WP254_88977 | wikipathways | 31 |
| Runx1 | AndrogenReceptor | netpath | 167 |
| Runx1 | Organic cation transport | reactome | 9 |
| Runx1 | RANKL | netpath | 84 |
| Runx1 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Runx1 | TGF_beta_Receptor | netpath | 220 |
| Runx1 | Validated transcriptional targets of deltaNp63 isoforms | pid | 47 |
| Runx1 | Pathways in cancer | kegg | 321 |
| Runx1 | Chronic myeloid leukemia | kegg | 69 |
| Runx1 | Acute myeloid leukemia | kegg | 53 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene