About Enhancer
| Enhancer ID: | E_02_0929 |
| Species: | human |
| Position : | chr6:161119825-161121825   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Hypoplasminogenemia |
| Pubmed ID: | 23872150 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,EMSA,PCR |
| Enhancer experiment description: | An AP3-like (activator protein 3) enhancer element, located 2.4 kb upstream of PLG-TSS, is involved in PLG transcriptional regulationIn summary we demonstrated that estrogen regulates plasminogen promoter activity. The 5-flanking region of the PLG gene con_x0002_tains several cis-acting elements that are responsive to estrogen.An enhancer located at -11.5 kb confers a dramatic estrogen re_x0002_sponse in reporter assays. |
About Target gene
| Target gene : | PLG(PLG) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,PCR |
| Target gene experiment description: | In our study we could show that a construct comprising the entire upstream region, containing the AP3 re_x0002_sponse element (2.4 kb-PLG) was able to further elevate basal luciferase activity 1.7-fold above that of PLG minimal promoters.The observed increase corresponds well to previously published results [20] and the 2.4 kb-PLG construct represents the most active contiguous promoter fragment analysed in this study. Surprisingly, with E2 stimulation this effect decreased by 54% down to the activity level of the PLG core promoter,although no ERE could be identified within this region. |
About TF
| TF name : | DHII(anon-EST:Posey114,anon-WO02059370.45,CG13094,DH31,DH-II,dh31,DH31,DH[31],DH[[31],Dh[[31]],DH[[31]],Dmel\CG13094,Drm-DH[[31]],drome-DH31,Drome-DH[[31]])ERE(ERE) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | Transcriptional effect of the DHII and/or ERE (_x0003_11.5 kb) enhancers on the heterologous crystallin promoter (cryst) driven luciferase activity in HepG2. |
About Function
| Enhancer function : | An AP3-like (activator protein 3) enhancer element, located 2.4 kb upstream of PLG-TSS, is involved in PLG transcriptional regulation. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
An AP3-like (activator protein 3) enhancer element, located 2.4 kb upstream of PLG-TSS, is involved in PLG transcriptional regulation [20]. In our study we could show that a construct comprising the entire upstream region, containing the AP3 response element (2.4 kb-PLG) was able to further elevate basal luciferase activity 1.7-fold above that of PLG minimal promoters. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene