About Enhancer
| Enhancer ID: | E_02_0930 |
| Species: | human |
| Position : | chr21:43770915-43772915   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 23675462 |
| Enhancer experiment: | RT-PCR,ChIP |
| Enhancer experiment description: | To determine whether MSK1 co-occupies the enhancer and UPE with H3S10ph, we performed sequential ChIP assays (re_x0002_ChIP) with mononucleosomes prepared from TPA- treated and formaldehyde-cross linked MCF-7 cell lysates.Together these results show that either MSK1 or MSK2 recruited to the TFF1 UPE and enhancer phopshorylates H3 at S10, leading to the binding of 14-3-3e/f and recruitment of BRG1. |
About Target gene
| Target gene : | TFF1(BCEI,D21S21,HP1.A,HPS2,pNR-2,pS2) |
| Strong evidence: | -- |
| Less strong evidence: | RT-PCR,ChIP |
| Target gene experiment description: | Here, we used the high-resolution chromatin immunoprecipi_x0002_tation (ChIP) assay to determine the MSK1, MSK2 and H3S10ph distribution along the regulatory and coding regions of the TFF1 gene in response to ERK-MAPK signaling.With this in mind, we performed ChIP assays with antibodies against H3S10phK14ac to determine the distribution of this dual H3 modification along the TFF1 gene._x0002_ |
About TF
| TF name : | RPS6KA5(MSK1,MSPK1,RLPK)RPS6KA4 (MSK2,RSK-B,S6K-alpha-4) |
| TF experiment: | ChIP |
| TF experiment description: | we used the high-resolution chromatin immunoprecipitation (ChIP) assay to determine the MSK1, MSK2 and H3S10ph distribution along the regulatory and coding regions of the TFF1 gene in response to ERK-MAPK signaling. |
About Function
| Enhancer function : | MSK1 and MSK2 belong to different multiprotein complexes but both mediate chromatin remodeling that is required at the Enhancer and UPE for TPA-induced initiation of TFF1 expression in MCF-7 breast cancer epithelial cells. |
| Enhancer function experiment: | Re-ChIP |
| Enhancer function experiment description: |
An AP3-like (activator protein 3) enhancer element, located 2.4 kb upstream of PLG-TSS, is involved in PLG transcriptional regulation [20]. In our study we could show that a construct comprising the entire upstream region, containing the AP3 response element (2.4 kb-PLG) was able to further elevate basal luciferase activity 1.7-fold above that of PLG minimal promoters. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene