About Enhancer
| Enhancer ID: | E_02_0934 |
| Species: | human |
| Position : | chr11:72897942-72912333   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 23636943 |
| Enhancer experiment: | 3C,ChIP-seq,ChIP-qPCR |
| Enhancer experiment description: | We integrated the data from our GRO-seq assays with data from a variety of other genomic assays (e.g., ChIP-seq, DNase-seq, ChIA-PET) using a novel computational pipeline to provide a comprehensive and global view of ESR1 enhancers and their regulation by E2 in MCF-7 cells. Together, our studies have shed new light on the activity of ESR1 at its enhancer sites and provide new insights about en_x0002_hancer function in general, including the potential roles of en_x0002_hancer transcription. |
About Target gene
| Target gene : | P2RY2(HP2U,P2RU1,P2U,P2U1,P2UR,P2Y2,P2Y2R) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-seq,ChIP-qPCR |
| Target gene experiment description: | As shown in the GRO-seq browser tracks in Figure 1A, transcription of the P2RY2 gene and a region around ERBS1 is up-regulated rapidly in a short time course of treatment with 17b-estradiol.The transcripts from ERBS1(Fig. 1A), as well as ERBSs 25 (Supplemental Fig. 2), are produced bidirectionally from both strands of DNA,reminiscent of the enhancer RNAs (eRNAs) described previously (Kim et al. 2010), and the transcribed regions are associated with RNA Pol II and pre_x0002_viously identified transcription start sites (TSSs). |
About TF
| TF name : | FOXA1(HNF3A,TCF3A) |
| TF experiment: | 3C-PCR |
| TF experiment description: | 3C-PCR assay showing E2-induced looping between ERBS1 and the P2RY2 gene. The lowercase letters correspond to the primers denoted by orange arrows shown in panel A. The assays were conducted in the presence (experimental) or absence (control) of DNA ligase, as indicated. Digested and ligated bacterial artificial chromosome (BAC) DNA spanning the entire P2YR2 locus was used as a PCR control. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| FOXA1 | AndrogenReceptor | netpath | 167 |
| FOXA1 | Direct p53 effectors | pid | 141 |
| FOXA1 | FOXA1 transcription factor network | pid | 45 |
| FOXA1 | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| FOXA1 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene