About Enhancer
| Enhancer ID: | E_02_0939 |
| Species: | mouse |
| Position : | chr17:48258757-48260757   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 23159876 |
| Enhancer experiment: | Luciferase Reporter Assay |
| Enhancer experiment description: | The Col1a1 promoter analysis showed the existence of two evolutionarily conserved regions containing pairs of Sp1 sites.These regions are located one at 1700 pb from the transcription start, known as bone enhancer(BE) and where the regulation of Col1a1 expression by Osx has been described,and another region located in the proximal promoter region. |
About Target gene
| Target gene : | COL1A1(EDSARTH1,EDSC,OI1,OI2,OI3,OI4) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,PCR |
| Target gene experiment description: | To further confirm the relevance of p38 in the regu_x0002_lation of Col1a1 by Osx, we studied the effect of a constitutive active form of MKK6, the MAPKK activating p38, combined with Osx on the ac_x0002_tivation of Col1a1 promoter by Osx. The overexpression of Osx or MKK6 activated both the full-length (?2483pCol1a1) as well the proximal promoter reporter constructs. Moreover, expression of Osx and MKK6 together had an additive effect on the activation of both reporter constructs. |
About TF
| TF name : | RUNX2(AML3,Cbf,Cbfa-1,Cbfa1,LS3,Osf2,PEBP2aA,Pebp2a1,Pebpa2a) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | Induction of long Osx transcriptional activity by Runx2. C2C12 cells were co-transfected with mock, short or long Osx constructs and/or Runx2 construct overnight, and serum-starved for 24 h. Luciferase activity was measured and normalized against ?-galactosidase activity. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| RUNX2 | AndrogenReceptor | netpath | 167 |
| RUNX2 | FGF signaling pathway | pid | 55 |
| RUNX2 | Leptin | netpath | 98 |
| RUNX2 | Notch-mediated HES/HEY network | pid | 48 |
| RUNX2 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| RUNX2 | Regulation of retinoblastoma protein | pid | 67 |
| RUNX2 | TGF_beta_Receptor | netpath | 220 |
| RUNX2 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| RUNX2 | Hs_MAPK_Signaling_Pathway_WP382_79951 | wikipathways | 58 |
| RUNX2 | Hs_Endochondral_Ossification_WP474_87977 | wikipathways | 43 |
| RUNX2 | Hs_Role_of_Osx_and_miRNAs_in_tooth_development_WP3971_91525 | wikipathways | 9 |
| RUNX2 | Hs_Bone_Morphogenic_Protein_(BMP)_Signalling_and_Regulation_WP1425_86082 | wikipathways | 6 |
| RUNX2 | Hs_Focal_Adhesion-PI3K-Akt-mTOR-signaling_pathway_WP3932_91101 | wikipathways | 8 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene