About Enhancer
| Enhancer ID: | E_02_0940 |
| Species: | human |
| Position : | chr11:69468761-69470761   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 23108396 |
| Enhancer experiment: | 3C,ChIP,qPCR |
| Enhancer experiment description: | ChIP confirmed that H2A.Z siRNA treatment lead to markedly reduced binding of H2A.Z to the CCND1 TSS. Concomitant to increased mRNA levels, polymerase II (pol II) recruitment to the CCND1 TSS slightly increased. |
About Target gene
| Target gene : | CCND1(BCL1,D11S287E,PRAD1,U21B31) |
| Strong evidence: | 3C |
| Less strong evidence: | qPCR |
| Target gene experiment description: | We conclude that enh2 has an active role in ER-independent CCND1 activation.To investigate whether the CCND1 TSS interacts with enh2, we used a chromatin conformation capture (3C) assay,which detects physical proximity between distal DNA sites by ligation of cross-linked restricted DNA fragments. |
About TF
| TF name : | -- |
| TF experiment: | -- |
| TF experiment description: | -- |
About Function
| Enhancer function : | The enh2 has an active role in ER-independent CCND1 activation. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
In addition, CCND1 activation in siH2AZ-transfected MDA-MB231 cells was accompanied by a strong recruitment of pol II to enh2 (Figure 1c, right panel), as described previously for E2 stimulated MCF-7 cells. We conclude that enh2 has an active role in ER-independent CCND1 activation. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene