About Enhancer
| Enhancer ID: | E_02_0942 |
| Species: | human |
| Position : | chr14:67743318-67743610   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Polycystic ovarian syndrome |
| Pubmed ID: | 22877652 |
| Enhancer experiment: | ChIP,PCR,Luciferase Reporter Assay,EMSA |
| Enhancer experiment description: | To determine whether AR interacts with endogenous chromatin in the GnRH-E1 region in GT1-7 cells, ChIP assays were performed. GT1-7 cells were transiently transfected with luciferase reporter constructs containing the GnRH-P (P), GnRH-E1 (E1), and/or GnRH-E2 (E2) regions, and/or the RSV promoter (RSVp) or enhancer (RSVe), as indicated, along with the AR expression vector. |
About Target gene
| Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay |
| Target gene experiment description: | AR associates with the enhancer 1 region of the GnRH regulatory region and represses transcription.GT1-7 cells were transiently transfected with luciferase reporter constructs containing the GnRH-P (P), GnRH-E1 (E1),and/or GnRH-E2 (E2) regions, and/or the RSV promoter (RSVp) or enhancer (RSVe), as indicated, along with the AR expression vector. |
About TF
| TF name : | Oct1Pbx(Pbx)Nkx2-1(BCH,BHC,NK-2,NKX2.1,NKX2A,NMTC1,T/EBP,TEBP,TITF1,TTF-1,TTF1) |
| TF experiment: | EMSA,PCR |
| TF experiment description: | AR bound to the -1796/-1791 sequence in electrophoretic mobility shift assays, indicating a direct interaction with DNA or other transcription factors in this region. |
About Function
| Enhancer function : | AR represses GnRH transcription via the major Enhancer (GnRH-E1). |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
For the rest of this study, we chose to evaluate the mechanism of repression by AR using GnRH-E1 upstream of RSVp to avoid com_x005f_x0002_pensation by the androgen-responsive GnRH promoter.To determine whether AR interacts with endogenous chromatin in the GnRH-E1 region in GT1-7 cells,ChIP assays were performed. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nkx2-1 | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| Nkx2-1 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene