About Enhancer

Enhancer ID: E_02_0943
Species: human
Position : chr8:124323890-124325890
Biosample name:
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: Prostate cancer
Pubmed ID:  22771493
Enhancer experiment: qPCR,Luciferase Reporter Assay,ChIP,EMSA,3C
Enhancer experiment description: Taken together, these data demonstrate that the poten_x0002_tial ARBS identified within the distal enhancer region of ATAD2 gene indeed interacts strongly and specifically with AR in vitro and suggest that it could be a good candidate as a functional ARBS in vivo.To ascertain whether the ARBS identified within the ATAD2 enhancer region is functional in cells in vivo, we performed ChIP using formaldehyde cross-linked LNCaP cells.

About Target gene

Target gene : ATAD2(ANCCA,CT137,PRO2000)
Strong evidence: --
Less strong evidence: ChIP,EMSA,RT-qPCR
Target gene experiment description: In addition, we analyzed chromatin immunoprecipita_x0002_tion (ChIP)-seq data from the literature to identify genes that might share the same regulation mechanism as ATAD2.With this in mind, we aimed first at checking whether ATAD2 might be a direct target gene of AR. To test this hypothesis, we analyzed ATAD2 gene response to androgens in LNCaP cells in the presence of cycloheximide (CHX), a protein synthesis inhibitor.

About TF

TF name : AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR)E2F1(E2F-1,RBAP1,RBBP3,RBP3)
TF experiment: EMSA
TF experiment description: To check the binding of AR to this putative ARBS, we first performed EMSA experiments with bacterially produced AR-DNA-binding domain (DBD) purified to homogeneity,siE2F1 and siAR transfection showed the very efficient and specific knockdown of E2F1 and AR, respectively, at both mRNA and protein levels.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
AR Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 reactome 65
AR Alpha6Beta4Integrin netpath 74
AR AndrogenReceptor netpath 167
AR Coregulation of Androgen receptor activity pid 61
AR FOXA1 transcription factor network pid 45
AR IL6 netpath 85
AR Nongenotropic Androgen signaling pid 31
AR Notch-mediated HES/HEY network pid 48
AR Nuclear Receptor transcription pathway reactome 51
AR Regulation of Androgen receptor activity pid 54
AR Regulation of nuclear beta catenin signaling and target gene transcription pid 80
AR Regulation of nuclear SMAD2/3 signaling pid 82
AR TGF_beta_Receptor netpath 220
AR Oocyte meiosis kegg 110
AR Pathways in cancer kegg 321
AR Prostate cancer kegg 85
AR Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
AR Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
AR Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
AR Hs_Sudden_Infant_Death_Syndrome_(SIDS)_Susceptibility_Pathways_WP706_86078 wikipathways 33
AR Hs_Androgen_receptor_signaling_pathway_WP138_79958 wikipathways 27
E2F1 Activation of PUMA and translocation to mitochondria reactome 8
E2F1 Association of licensing factors with the pre-replicative complex reactome 15
E2F1 Calcineurin-regulated NFAT-dependent transcription in lymphocytes pid 50
E2F1 CDC6 association with the ORC:origin complex reactome 11
E2F1 Cyclin D associated events in G1 reactome 38
E2F1 Direct p53 effectors pid 141
E2F1 E2F mediated regulation of DNA replication reactome 17
E2F1 E2F transcription factor network pid 77
E2F1 G0 and Early G1 reactome 25
E2F1 G1/S-Specific Transcription reactome 17
E2F1 G2 Phase reactome 5
E2F1 IL2 signaling events mediated by PI3K pid 38
E2F1 Inhibition of replication initiation of damaged DNA by RB1/E2F1 reactome 12
E2F1 Notch-mediated HES/HEY network pid 48
E2F1 Oncogene Induced Senescence reactome 30
E2F1 Oxidative Stress Induced Senescence reactome 120
E2F1 p75(NTR)-mediated signaling pid 74
E2F1 Pre-NOTCH Transcription and Translation reactome 29
E2F1 Regulation of retinoblastoma protein pid 67
E2F1 Regulation of Telomerase pid 70
E2F1 TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest reactome 13
E2F1 Cell cycle kegg 124
E2F1 Pathways in cancer kegg 321
E2F1 Pancreatic cancer kegg 70
E2F1 Glioma kegg 65
E2F1 Prostate cancer kegg 85
E2F1 Melanoma kegg 69
E2F1 Bladder cancer kegg 38
E2F1 Chronic myeloid leukemia kegg 69
E2F1 Small cell lung cancer kegg 83
E2F1 Non-small cell lung cancer kegg 54
E2F1 Hs_DNA_Damage_Response_WP707_82937 wikipathways 28
E2F1 Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694 wikipathways 28
E2F1 Hs_miRNAs_involved_in_DNA_damage_response_WP1545_84697 wikipathways 30
E2F1 Hs_Endochondral_Ossification_WP474_87977 wikipathways 43
E2F1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
E2F1 Hs_Integrated_Cancer_Pathway_WP1971_82939 wikipathways 33
E2F1 Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367 wikipathways 50
E2F1 Hs_Bladder_Cancer_WP2828_89143 wikipathways 17
E2F1 Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520 wikipathways 16
E2F1 Hs_Aryl_Hydrocarbon_Receptor_WP2586_91687 wikipathways 16
E2F1 Hs_Fatty_Acid_Beta_Oxidation_WP143_79783 wikipathways 14
E2F1 Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs