About Enhancer
| Enhancer ID: | E_02_0944 |
| Species: | mouse |
| Position : | chr14:67741735-67744661   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 22761896 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,PCR |
| Enhancer experiment description: | the luciferase activities in GT17 cells were noticeably decreased without HDACIs treatments, suggesting that potential enhancer elements located in the regions (-3446,-2087 bp and -1134,-520 bp). |
About Target gene
| Target gene : | Gnrh1(Gnrh,Gnrh2,LHRH,Lhrh1,Lnrh,hpg) |
| Strong evidence: | -- |
| Less strong evidence: | Western blot,Immunofluorescence,ChIP,EMSA |
| Target gene experiment description: | The Otx2 protein levels were also obviously reduced in GT17 cells showed by western blot analysis (Figure 5B) and immunofluorescence staining (Figure 5C).The results of ChIP assay showed that the binding ability of Otx2 to neuron-specific elements (2356,2249 bp) in Gnrh1 promoter obviously decreased after HDACIs treatments (Figure 6B).To further analyze the affinities of Otx2 to the two conserved binding sites in mouse Gnrh1 promoter respectively, EMSA was performed with probes containing the 2268,2239 bp and 2330,2301 bp sequences [20](Figure 6C, above). |
About TF
| TF name : | Otx2(E130306E05Rik) |
| TF experiment: | ChIP,EMSA |
| TF experiment description: | Chromatin immunoprecipitation and electrophoretic mobility shift assays demonstrated that the DNA-binding activity of Otx2 to Gnrh1 gene was suppressed by TSA and VPA. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene