About Enhancer
| Enhancer ID: | E_02_0946 |
| Species: | mouse |
| Position : | chr14:47056226-47056692   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 22701669 |
| Enhancer experiment: | EMSA,Phylogenetic Footprinting |
| Enhancer experiment description: | In vitro electrophoretic mobility shift assays (EMSA) and in vivo transgenic reporter mutational analyses revealed that this site supports Pitx binding and that the site is necessary to recapitulate aspects of endogenous Bmp4 expression in developing craniofacial and limb tissues. |
About Target gene
| Target gene : | Bmp4(Atoh5,Math4B,bHLHa7,ngn3) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,PCR |
| Target gene experiment description: | Pitx2 chromatin immunoprecipitation (ChIP) demonstrate direct binding of Pitx2 to this Bmp4 enhancer site in a dental epithelial cell line.Using primers that target the Pitx1/2 binding motif in the 396 bp Bmp4 enhancer sequence (CONS3.8), DNA purified from crosslinked LS8 chromatin after immunoprecipitation with an anti-Pitx2 antibody yielded a 4.7-fold increase in amplicon abundance, relative to an IgG control, by PCR and qPCR (Figure 6A, B). |
About TF
| TF name : | Pitx2(9430085M16Rik,Brx1,Brx1b,Munc30,Otlx2,Ptx2,Rieg) |
| TF experiment: | EMSA,ChIP |
| TF experiment description: | Electrophoretic Mobility Shift Assay (EMSA) exhibits robust binding of Pitx1 protein to both a positive control bicoid DNA sequence and to the consensus Pitx1/2 binding site in with a 25 probe sequence in the CONS3.8 sequenceTo determine whether Pitx2 physically binds the CONS3.8 Bmp4 Enhancer in living cells,we performed Chromatin Immunoprecipitation (ChIP) assays in LS8 mouse dental epithelial cells. |
About Function
| Enhancer function : | Pitx2 ChIP demonstrated direct binding of Pitx2 to this Bmp4 enhancer site in a dental epithelial cell line. |
| Enhancer function experiment: | ChIP,PCR,qPCR |
| Enhancer function experiment description: |
Using primers that target the Pitx1/2 binding motif in the 396 bp Bmp4 Enhancer sequence (CONS3.8), DNA purified from crosslinked LS8 chromatin after immunoprecipitation with an anti-Pitx2 antibody yielded a 4.7-fold increase in amplicon abundance, relative to an IgG control, by PCR and qPCR. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Pitx2 | Regulation of nuclear beta catenin signaling and target gene transcription | pid | 80 |
| Pitx2 | TGF-beta signaling pathway | kegg | 82 |
| Pitx2 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| Pitx2 | Hs_BMP_Signaling_Pathway_in_Eyelid_Development_WP3927_90735 | wikipathways | 11 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene