About Enhancer
| Enhancer ID: | E_02_0947 |
| Species: | human |
| Position : | chr17:69107686-69108045   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 22665440 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,3C-qPCR,ChIP-qPCR |
| Enhancer experiment description: | To determine if the rs1859962 PCa risk LD block harbors enhancers, we examined the H3K4me1 ge_x0002_nome-wide distribution defined by ChIP-seq assays in LNCaP and VCaP PCa cells.We show that there are five enhancers within the target genomic segment, hereafter referred to as E1E5. |
About Target gene
| Target gene : | SOX9(CMD1,CMPD1,SRA1,SRXX2,SRXY10) |
| Strong evidence: | -- |
| Less strong evidence: | qPCR |
| Target gene experiment description: | This chromatin loop was confirmed through TaqMan probebased qPCR assays with a probe targeting the SOX9 gene (Fig. 2C). The chromatin interaction was further confirmed by sequencing the PCR product (Supplemental Fig. S7). Further_x0002_more, this chromatin loop appears to be specific to SOX9, because no other loop could be detected between E1 and any other genes(MAP2K6, KCNJ16, KCNJ2) found within this ;3-Mb window. |
About TF
| TF name : | AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR)FOXA1(HNF3A,TCF3A) |
| TF experiment: | ChIP,Luciferase Reporter Assay |
| TF experiment description: | In agreement, the luciferase assay in LNCaP cells depletedor not of AR using siRNA reveals that AR is required for the increased transcriptional response associated with the rs8072254 variant A allele.The FKH PWM, defined based on thousands of FOXA1 binding sites identified by ChIP-seq assays, reveals that the tenth position is invariably an A residue. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | rs8072254 |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene